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Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay

Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated ins...

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Autores principales: Zhang, Chao, Liu, Xiaonan, Yao, Yao, Liu, Kewu, Hui, Wenli, Zhu, Juanli, Dou, Yaling, Hua, Kai, Peng, Mingli, Wang, Zuankai, Vermorken, Alphonsus J.M., Cui, Yali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153416/
https://www.ncbi.nlm.nih.gov/pubmed/30245369
http://dx.doi.org/10.1016/j.isci.2018.09.005
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author Zhang, Chao
Liu, Xiaonan
Yao, Yao
Liu, Kewu
Hui, Wenli
Zhu, Juanli
Dou, Yaling
Hua, Kai
Peng, Mingli
Wang, Zuankai
Vermorken, Alphonsus J.M.
Cui, Yali
author_facet Zhang, Chao
Liu, Xiaonan
Yao, Yao
Liu, Kewu
Hui, Wenli
Zhu, Juanli
Dou, Yaling
Hua, Kai
Peng, Mingli
Wang, Zuankai
Vermorken, Alphonsus J.M.
Cui, Yali
author_sort Zhang, Chao
collection PubMed
description Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA) system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2) can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 10(2) cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms.
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spelling pubmed-61534162018-09-25 Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay Zhang, Chao Liu, Xiaonan Yao, Yao Liu, Kewu Hui, Wenli Zhu, Juanli Dou, Yaling Hua, Kai Peng, Mingli Wang, Zuankai Vermorken, Alphonsus J.M. Cui, Yali iScience Article Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA) system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2) can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 10(2) cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms. Elsevier 2018-09-08 /pmc/articles/PMC6153416/ /pubmed/30245369 http://dx.doi.org/10.1016/j.isci.2018.09.005 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Chao
Liu, Xiaonan
Yao, Yao
Liu, Kewu
Hui, Wenli
Zhu, Juanli
Dou, Yaling
Hua, Kai
Peng, Mingli
Wang, Zuankai
Vermorken, Alphonsus J.M.
Cui, Yali
Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
title Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
title_full Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
title_fullStr Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
title_full_unstemmed Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
title_short Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
title_sort genotyping of multiple clinical samples with a combined direct pcr and magnetic lateral flow assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153416/
https://www.ncbi.nlm.nih.gov/pubmed/30245369
http://dx.doi.org/10.1016/j.isci.2018.09.005
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