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Alterations of MicroRNA Expression in the Liver, Heart, and Testis of Mice Upon Exposure to Repeated Low-Dose Radiation
MicroRNAs (miRs), which regulate target gene expression at the post-transcriptional level, play a crucial role in inducing biological effects upon high-dose ionizing radiation. Yet, the miR expression profiles in response to repeated low-dose radiation (LDR) in vivo have not been elucidated. This st...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153535/ https://www.ncbi.nlm.nih.gov/pubmed/30263020 http://dx.doi.org/10.1177/1559325818799561 |
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author | Liang, Xinyue Zheng, Shirong Cui, Jiuwei Yu, Dehai Yang, Guozi Zhou, Lei Wang, Brain Cai, Lu Li, Wei |
author_facet | Liang, Xinyue Zheng, Shirong Cui, Jiuwei Yu, Dehai Yang, Guozi Zhou, Lei Wang, Brain Cai, Lu Li, Wei |
author_sort | Liang, Xinyue |
collection | PubMed |
description | MicroRNAs (miRs), which regulate target gene expression at the post-transcriptional level, play a crucial role in inducing biological effects upon high-dose ionizing radiation. Yet, the miR expression profiles in response to repeated low-dose radiation (LDR) in vivo have not been elucidated. This study investigated the response profiles of 11 miRs with functions involved in metabolism, DNA damage and repair, inflammation, and fibrosis in mouse liver, heart, and testis upon repeated LDR exposure for 4 months. The expression profiles were evaluated using stem-loop quantitative reverse transcription polymerase chain reaction immediately and at 2 months after LDR exposure. The expression profiles varied significantly at both time points. At the organ level, the heart was the most affected, followed by the liver and testis, in which significant miR upregulation related to DNA damage response was found. Metabolism-related miRs decreased in the liver and increased in the testis. The current results showed immediate and long-lasting alterations in the miR expression profiles in response to repeated LDR in different organs. |
format | Online Article Text |
id | pubmed-6153535 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-61535352018-09-27 Alterations of MicroRNA Expression in the Liver, Heart, and Testis of Mice Upon Exposure to Repeated Low-Dose Radiation Liang, Xinyue Zheng, Shirong Cui, Jiuwei Yu, Dehai Yang, Guozi Zhou, Lei Wang, Brain Cai, Lu Li, Wei Dose Response Potential Clinical Implication of LDR Hormesis and Adaptive Response MicroRNAs (miRs), which regulate target gene expression at the post-transcriptional level, play a crucial role in inducing biological effects upon high-dose ionizing radiation. Yet, the miR expression profiles in response to repeated low-dose radiation (LDR) in vivo have not been elucidated. This study investigated the response profiles of 11 miRs with functions involved in metabolism, DNA damage and repair, inflammation, and fibrosis in mouse liver, heart, and testis upon repeated LDR exposure for 4 months. The expression profiles were evaluated using stem-loop quantitative reverse transcription polymerase chain reaction immediately and at 2 months after LDR exposure. The expression profiles varied significantly at both time points. At the organ level, the heart was the most affected, followed by the liver and testis, in which significant miR upregulation related to DNA damage response was found. Metabolism-related miRs decreased in the liver and increased in the testis. The current results showed immediate and long-lasting alterations in the miR expression profiles in response to repeated LDR in different organs. SAGE Publications 2018-09-24 /pmc/articles/PMC6153535/ /pubmed/30263020 http://dx.doi.org/10.1177/1559325818799561 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Potential Clinical Implication of LDR Hormesis and Adaptive Response Liang, Xinyue Zheng, Shirong Cui, Jiuwei Yu, Dehai Yang, Guozi Zhou, Lei Wang, Brain Cai, Lu Li, Wei Alterations of MicroRNA Expression in the Liver, Heart, and Testis of Mice Upon Exposure to Repeated Low-Dose Radiation |
title | Alterations of MicroRNA Expression in the Liver, Heart, and Testis of
Mice Upon Exposure to Repeated Low-Dose Radiation |
title_full | Alterations of MicroRNA Expression in the Liver, Heart, and Testis of
Mice Upon Exposure to Repeated Low-Dose Radiation |
title_fullStr | Alterations of MicroRNA Expression in the Liver, Heart, and Testis of
Mice Upon Exposure to Repeated Low-Dose Radiation |
title_full_unstemmed | Alterations of MicroRNA Expression in the Liver, Heart, and Testis of
Mice Upon Exposure to Repeated Low-Dose Radiation |
title_short | Alterations of MicroRNA Expression in the Liver, Heart, and Testis of
Mice Upon Exposure to Repeated Low-Dose Radiation |
title_sort | alterations of microrna expression in the liver, heart, and testis of
mice upon exposure to repeated low-dose radiation |
topic | Potential Clinical Implication of LDR Hormesis and Adaptive Response |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153535/ https://www.ncbi.nlm.nih.gov/pubmed/30263020 http://dx.doi.org/10.1177/1559325818799561 |
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