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Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors

The use of short-wave infrared (SWIR) light for fluorescence bioimaging offers the advantage of reduced photon scattering and improved tissue penetration compared to traditional shorter wavelength imaging approaches. While several nanomaterials have been shown capable of generating SWIR emissions, r...

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Autores principales: Naczynski, Dominik Jan, Stafford, Jason H., Türkcan, Silvan, Jenkins, Cesare, Koh, Ai Leen, Sun, Conroy, Xing, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153542/
https://www.ncbi.nlm.nih.gov/pubmed/30246593
http://dx.doi.org/10.1177/1536012118799131
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author Naczynski, Dominik Jan
Stafford, Jason H.
Türkcan, Silvan
Jenkins, Cesare
Koh, Ai Leen
Sun, Conroy
Xing, Lei
author_facet Naczynski, Dominik Jan
Stafford, Jason H.
Türkcan, Silvan
Jenkins, Cesare
Koh, Ai Leen
Sun, Conroy
Xing, Lei
author_sort Naczynski, Dominik Jan
collection PubMed
description The use of short-wave infrared (SWIR) light for fluorescence bioimaging offers the advantage of reduced photon scattering and improved tissue penetration compared to traditional shorter wavelength imaging approaches. While several nanomaterials have been shown capable of generating SWIR emissions, rare-earth-doped nanoparticles (REs) have emerged as an exceptionally bright and biocompatible class of SWIR emitters. Here, we demonstrate SWIR imaging of REs for several applications, including lymphatic mapping, real-time monitoring of probe biodistribution, and molecular targeting of the α(v)β(3) integrin in a tumor model. We further quantified the resolution and depth penetration limits of SWIR light emitted by REs in a customized imaging unit engineered for SWIR imaging of live small animals. Our results indicate that SWIR light has broad utility for preclinical biomedical imaging and demonstrates the potential for molecular imaging using targeted REs.
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spelling pubmed-61535422018-09-27 Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors Naczynski, Dominik Jan Stafford, Jason H. Türkcan, Silvan Jenkins, Cesare Koh, Ai Leen Sun, Conroy Xing, Lei Mol Imaging Brief Report The use of short-wave infrared (SWIR) light for fluorescence bioimaging offers the advantage of reduced photon scattering and improved tissue penetration compared to traditional shorter wavelength imaging approaches. While several nanomaterials have been shown capable of generating SWIR emissions, rare-earth-doped nanoparticles (REs) have emerged as an exceptionally bright and biocompatible class of SWIR emitters. Here, we demonstrate SWIR imaging of REs for several applications, including lymphatic mapping, real-time monitoring of probe biodistribution, and molecular targeting of the α(v)β(3) integrin in a tumor model. We further quantified the resolution and depth penetration limits of SWIR light emitted by REs in a customized imaging unit engineered for SWIR imaging of live small animals. Our results indicate that SWIR light has broad utility for preclinical biomedical imaging and demonstrates the potential for molecular imaging using targeted REs. SAGE Publications 2018-09-24 /pmc/articles/PMC6153542/ /pubmed/30246593 http://dx.doi.org/10.1177/1536012118799131 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Brief Report
Naczynski, Dominik Jan
Stafford, Jason H.
Türkcan, Silvan
Jenkins, Cesare
Koh, Ai Leen
Sun, Conroy
Xing, Lei
Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors
title Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors
title_full Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors
title_fullStr Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors
title_full_unstemmed Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors
title_short Rare-Earth-Doped Nanoparticles for Short-Wave Infrared Fluorescence Bioimaging and Molecular Targeting of α(V)β(3)-Expressing Tumors
title_sort rare-earth-doped nanoparticles for short-wave infrared fluorescence bioimaging and molecular targeting of α(v)β(3)-expressing tumors
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153542/
https://www.ncbi.nlm.nih.gov/pubmed/30246593
http://dx.doi.org/10.1177/1536012118799131
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