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The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy

Photoacoustic microscopy is dominantly sensitive to the endogenous optical absorption, while a fluorescence optical microscopy can detect the fluorescence emission to obtain the image of a sample. To some extent, the physical processes of the 2 methods are opposite, one is absorption and another is...

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Autores principales: Zhang, Yu, Tang, Zhilie, Wu, Yongbo, Xue, Yueju, Jia, Jinliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153544/
https://www.ncbi.nlm.nih.gov/pubmed/30249167
http://dx.doi.org/10.1177/1533033818793424
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author Zhang, Yu
Tang, Zhilie
Wu, Yongbo
Xue, Yueju
Jia, Jinliang
author_facet Zhang, Yu
Tang, Zhilie
Wu, Yongbo
Xue, Yueju
Jia, Jinliang
author_sort Zhang, Yu
collection PubMed
description Photoacoustic microscopy is dominantly sensitive to the endogenous optical absorption, while a fluorescence optical microscopy can detect the fluorescence emission to obtain the image of a sample. To some extent, the physical processes of the 2 methods are opposite, one is absorption and another is emission, but both can be used to image cells. In this article, a simultaneous dual-mode imaging system of photoacoustic microscopy and fluorescence optical microscopy is set up to image tobacco cells. Furthermore, gold nanoparticles, which have a large absorption coefficient and enough fluorescence emission with wavelength of 512 nm, are used to label certain drugs and added to the tobacco cells. Then based on the simultaneous dual-mode microscopy imaging system, the photoacoustic microscopy and fluorescence optical microscopy images of gold nanoparticle-labeled tobacco cells are obtained. The final purpose of this experimental research is to detect if the labeled drugs can enter the cells by the positions of the gold nanoparticles. This will help the experts to deliver organic pesticide more accurately and effectively. The experimental results show that by gold nanoparticle labeling technology, the imaging quality of photoacoustic microscopy and fluorescence optical microscopy can be improved, which indicates that the drugs probably enter the tobacco cells successfully.
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spelling pubmed-61535442018-09-27 The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy Zhang, Yu Tang, Zhilie Wu, Yongbo Xue, Yueju Jia, Jinliang Technol Cancer Res Treat Original Article Photoacoustic microscopy is dominantly sensitive to the endogenous optical absorption, while a fluorescence optical microscopy can detect the fluorescence emission to obtain the image of a sample. To some extent, the physical processes of the 2 methods are opposite, one is absorption and another is emission, but both can be used to image cells. In this article, a simultaneous dual-mode imaging system of photoacoustic microscopy and fluorescence optical microscopy is set up to image tobacco cells. Furthermore, gold nanoparticles, which have a large absorption coefficient and enough fluorescence emission with wavelength of 512 nm, are used to label certain drugs and added to the tobacco cells. Then based on the simultaneous dual-mode microscopy imaging system, the photoacoustic microscopy and fluorescence optical microscopy images of gold nanoparticle-labeled tobacco cells are obtained. The final purpose of this experimental research is to detect if the labeled drugs can enter the cells by the positions of the gold nanoparticles. This will help the experts to deliver organic pesticide more accurately and effectively. The experimental results show that by gold nanoparticle labeling technology, the imaging quality of photoacoustic microscopy and fluorescence optical microscopy can be improved, which indicates that the drugs probably enter the tobacco cells successfully. SAGE Publications 2018-09-24 /pmc/articles/PMC6153544/ /pubmed/30249167 http://dx.doi.org/10.1177/1533033818793424 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Zhang, Yu
Tang, Zhilie
Wu, Yongbo
Xue, Yueju
Jia, Jinliang
The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
title The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
title_full The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
title_fullStr The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
title_full_unstemmed The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
title_short The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
title_sort dual-mode imaging of nanogold-labeled cells by photoacoustic microscopy and fluorescence optical microscopy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153544/
https://www.ncbi.nlm.nih.gov/pubmed/30249167
http://dx.doi.org/10.1177/1533033818793424
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