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Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of i...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153728/ https://www.ncbi.nlm.nih.gov/pubmed/30011025 http://dx.doi.org/10.1093/intimm/dxy046 |
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author | Nagatake, Takahiro Suzuki, Hidehiko Hirata, So-ichiro Matsumoto, Naomi Wada, Yasuko Morimoto, Sakiko Nasu, Ayaka Shimojou, Michiko Kawano, Mitsuo Ogami, Kentaro Tsujimura, Yusuke Kuroda, Etsushi Iijima, Norifumi Hosomi, Koji Ishii, Ken J Nosaka, Tetsuya Yasutomi, Yasuhiro Kunisawa, Jun |
author_facet | Nagatake, Takahiro Suzuki, Hidehiko Hirata, So-ichiro Matsumoto, Naomi Wada, Yasuko Morimoto, Sakiko Nasu, Ayaka Shimojou, Michiko Kawano, Mitsuo Ogami, Kentaro Tsujimura, Yusuke Kuroda, Etsushi Iijima, Norifumi Hosomi, Koji Ishii, Ken J Nosaka, Tetsuya Yasutomi, Yasuhiro Kunisawa, Jun |
author_sort | Nagatake, Takahiro |
collection | PubMed |
description | We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of inducible bronchus-associated lymphoid tissue (iBALT) played a role in the induction of antigen-specific T cells and IgA antibody responses in the lung of mice intra-nasally administered Ag85B-rHPIV2. We found that expression of Ag85B was dispensable for the development of iBALT, suggesting that HPIV2 acted as an iBALT-inducing vector. When iBALT organogenesis was disrupted in Ag85B-rHPIV2-immunized mice, either by neutralization of the lymphotoxin pathway or depletion of CD11b(+) cells, Ag85B-specific immune responses (i.e. IFN γ-producing T cells and IgA antibody) were diminished in the lung. Furthermore, we found that immunization with Ag85B-rHPIV2 induced neutrophil and eosinophil infiltration temporally after the immunization in the lung. Thus, our results show that iBALT organogenesis contributes to the induction of antigen-specific immune responses by Ag85B-rHPIV2 and that Ag85B-rHPIV2 provokes its immune responses without inducing long-lasting inflammation. |
format | Online Article Text |
id | pubmed-6153728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61537282018-09-28 Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice Nagatake, Takahiro Suzuki, Hidehiko Hirata, So-ichiro Matsumoto, Naomi Wada, Yasuko Morimoto, Sakiko Nasu, Ayaka Shimojou, Michiko Kawano, Mitsuo Ogami, Kentaro Tsujimura, Yusuke Kuroda, Etsushi Iijima, Norifumi Hosomi, Koji Ishii, Ken J Nosaka, Tetsuya Yasutomi, Yasuhiro Kunisawa, Jun Int Immunol Featured Article of the Month We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of inducible bronchus-associated lymphoid tissue (iBALT) played a role in the induction of antigen-specific T cells and IgA antibody responses in the lung of mice intra-nasally administered Ag85B-rHPIV2. We found that expression of Ag85B was dispensable for the development of iBALT, suggesting that HPIV2 acted as an iBALT-inducing vector. When iBALT organogenesis was disrupted in Ag85B-rHPIV2-immunized mice, either by neutralization of the lymphotoxin pathway or depletion of CD11b(+) cells, Ag85B-specific immune responses (i.e. IFN γ-producing T cells and IgA antibody) were diminished in the lung. Furthermore, we found that immunization with Ag85B-rHPIV2 induced neutrophil and eosinophil infiltration temporally after the immunization in the lung. Thus, our results show that iBALT organogenesis contributes to the induction of antigen-specific immune responses by Ag85B-rHPIV2 and that Ag85B-rHPIV2 provokes its immune responses without inducing long-lasting inflammation. Oxford University Press 2018-07-16 /pmc/articles/PMC6153728/ /pubmed/30011025 http://dx.doi.org/10.1093/intimm/dxy046 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society for Immunology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Featured Article of the Month Nagatake, Takahiro Suzuki, Hidehiko Hirata, So-ichiro Matsumoto, Naomi Wada, Yasuko Morimoto, Sakiko Nasu, Ayaka Shimojou, Michiko Kawano, Mitsuo Ogami, Kentaro Tsujimura, Yusuke Kuroda, Etsushi Iijima, Norifumi Hosomi, Koji Ishii, Ken J Nosaka, Tetsuya Yasutomi, Yasuhiro Kunisawa, Jun Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
title | Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
title_full | Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
title_fullStr | Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
title_full_unstemmed | Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
title_short | Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
title_sort | immunological association of inducible bronchus-associated lymphoid tissue organogenesis in ag85b-rhpiv2 vaccine-induced anti-tuberculosis mucosal immune responses in mice |
topic | Featured Article of the Month |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153728/ https://www.ncbi.nlm.nih.gov/pubmed/30011025 http://dx.doi.org/10.1093/intimm/dxy046 |
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