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Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice

We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of i...

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Autores principales: Nagatake, Takahiro, Suzuki, Hidehiko, Hirata, So-ichiro, Matsumoto, Naomi, Wada, Yasuko, Morimoto, Sakiko, Nasu, Ayaka, Shimojou, Michiko, Kawano, Mitsuo, Ogami, Kentaro, Tsujimura, Yusuke, Kuroda, Etsushi, Iijima, Norifumi, Hosomi, Koji, Ishii, Ken J, Nosaka, Tetsuya, Yasutomi, Yasuhiro, Kunisawa, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153728/
https://www.ncbi.nlm.nih.gov/pubmed/30011025
http://dx.doi.org/10.1093/intimm/dxy046
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author Nagatake, Takahiro
Suzuki, Hidehiko
Hirata, So-ichiro
Matsumoto, Naomi
Wada, Yasuko
Morimoto, Sakiko
Nasu, Ayaka
Shimojou, Michiko
Kawano, Mitsuo
Ogami, Kentaro
Tsujimura, Yusuke
Kuroda, Etsushi
Iijima, Norifumi
Hosomi, Koji
Ishii, Ken J
Nosaka, Tetsuya
Yasutomi, Yasuhiro
Kunisawa, Jun
author_facet Nagatake, Takahiro
Suzuki, Hidehiko
Hirata, So-ichiro
Matsumoto, Naomi
Wada, Yasuko
Morimoto, Sakiko
Nasu, Ayaka
Shimojou, Michiko
Kawano, Mitsuo
Ogami, Kentaro
Tsujimura, Yusuke
Kuroda, Etsushi
Iijima, Norifumi
Hosomi, Koji
Ishii, Ken J
Nosaka, Tetsuya
Yasutomi, Yasuhiro
Kunisawa, Jun
author_sort Nagatake, Takahiro
collection PubMed
description We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of inducible bronchus-associated lymphoid tissue (iBALT) played a role in the induction of antigen-specific T cells and IgA antibody responses in the lung of mice intra-nasally administered Ag85B-rHPIV2. We found that expression of Ag85B was dispensable for the development of iBALT, suggesting that HPIV2 acted as an iBALT-inducing vector. When iBALT organogenesis was disrupted in Ag85B-rHPIV2-immunized mice, either by neutralization of the lymphotoxin pathway or depletion of CD11b(+) cells, Ag85B-specific immune responses (i.e. IFN γ-producing T cells and IgA antibody) were diminished in the lung. Furthermore, we found that immunization with Ag85B-rHPIV2 induced neutrophil and eosinophil infiltration temporally after the immunization in the lung. Thus, our results show that iBALT organogenesis contributes to the induction of antigen-specific immune responses by Ag85B-rHPIV2 and that Ag85B-rHPIV2 provokes its immune responses without inducing long-lasting inflammation.
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spelling pubmed-61537282018-09-28 Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice Nagatake, Takahiro Suzuki, Hidehiko Hirata, So-ichiro Matsumoto, Naomi Wada, Yasuko Morimoto, Sakiko Nasu, Ayaka Shimojou, Michiko Kawano, Mitsuo Ogami, Kentaro Tsujimura, Yusuke Kuroda, Etsushi Iijima, Norifumi Hosomi, Koji Ishii, Ken J Nosaka, Tetsuya Yasutomi, Yasuhiro Kunisawa, Jun Int Immunol Featured Article of the Month We previously reported that Ag85B-expressing human parainfluenza type 2 virus (Ag85B-rHPIV2) was effective as a nasal vaccine against tuberculosis in mice; however, the mechanism by which it induces an immune response remains to be investigated. In the present study, we found that organogenesis of inducible bronchus-associated lymphoid tissue (iBALT) played a role in the induction of antigen-specific T cells and IgA antibody responses in the lung of mice intra-nasally administered Ag85B-rHPIV2. We found that expression of Ag85B was dispensable for the development of iBALT, suggesting that HPIV2 acted as an iBALT-inducing vector. When iBALT organogenesis was disrupted in Ag85B-rHPIV2-immunized mice, either by neutralization of the lymphotoxin pathway or depletion of CD11b(+) cells, Ag85B-specific immune responses (i.e. IFN γ-producing T cells and IgA antibody) were diminished in the lung. Furthermore, we found that immunization with Ag85B-rHPIV2 induced neutrophil and eosinophil infiltration temporally after the immunization in the lung. Thus, our results show that iBALT organogenesis contributes to the induction of antigen-specific immune responses by Ag85B-rHPIV2 and that Ag85B-rHPIV2 provokes its immune responses without inducing long-lasting inflammation. Oxford University Press 2018-07-16 /pmc/articles/PMC6153728/ /pubmed/30011025 http://dx.doi.org/10.1093/intimm/dxy046 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society for Immunology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Featured Article of the Month
Nagatake, Takahiro
Suzuki, Hidehiko
Hirata, So-ichiro
Matsumoto, Naomi
Wada, Yasuko
Morimoto, Sakiko
Nasu, Ayaka
Shimojou, Michiko
Kawano, Mitsuo
Ogami, Kentaro
Tsujimura, Yusuke
Kuroda, Etsushi
Iijima, Norifumi
Hosomi, Koji
Ishii, Ken J
Nosaka, Tetsuya
Yasutomi, Yasuhiro
Kunisawa, Jun
Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
title Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
title_full Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
title_fullStr Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
title_full_unstemmed Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
title_short Immunological association of inducible bronchus-associated lymphoid tissue organogenesis in Ag85B-rHPIV2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
title_sort immunological association of inducible bronchus-associated lymphoid tissue organogenesis in ag85b-rhpiv2 vaccine-induced anti-tuberculosis mucosal immune responses in mice
topic Featured Article of the Month
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153728/
https://www.ncbi.nlm.nih.gov/pubmed/30011025
http://dx.doi.org/10.1093/intimm/dxy046
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