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Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats

CONTEXT: Makaradhwaja is a Kupipakwa Rasayana. Since it contains two heavy metals, namely mercury and gold, it is essential to evaluate its safety. Hence, the present study was undertaken with an objective to evaluate toxicity and target organ of toxicity of Makaradhwaja if so. AIMS: The objective w...

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Autores principales: Jamadagni, Shrirang, Jamadagni, Pallavi Shrirang, Singh, Rajendra Kumar, Upadhyay, Sachchidanand, Gaidhani, Sudesh N., Hazra, Jayram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153918/
https://www.ncbi.nlm.nih.gov/pubmed/30254400
http://dx.doi.org/10.4103/ayu.AYU_33_17
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author Jamadagni, Shrirang
Jamadagni, Pallavi Shrirang
Singh, Rajendra Kumar
Upadhyay, Sachchidanand
Gaidhani, Sudesh N.
Hazra, Jayram
author_facet Jamadagni, Shrirang
Jamadagni, Pallavi Shrirang
Singh, Rajendra Kumar
Upadhyay, Sachchidanand
Gaidhani, Sudesh N.
Hazra, Jayram
author_sort Jamadagni, Shrirang
collection PubMed
description CONTEXT: Makaradhwaja is a Kupipakwa Rasayana. Since it contains two heavy metals, namely mercury and gold, it is essential to evaluate its safety. Hence, the present study was undertaken with an objective to evaluate toxicity and target organ of toxicity of Makaradhwaja if so. AIMS: The objective was to evaluate toxicological profile, the target organ of toxicity and to find no observed effect level (NOEL) or no observed adverse effect level (NOAEL) in rats after oral administration for ninety consecutive days. MATERIALS AND METHODS: Makaradhawaja preparation was administered to male and female Wistar rats for ninety consecutive days at 2.7, 13.5, and 27 mg/kg body weight. All relevant biochemical and hematological changes were observed. At termination, all the rats were sacrificed and necropsy was performed. Histopathological evaluation was also performed. STATISTICAL ANALYSIS USED: Dunnett's test followed by analysis of variance. RESULTS: There was a significant increase in high-dose group kidney weight of both sexes which could not be correlated with histopathology findings and serum biochemistry. Therefore, the change was not considered as an adverse effect. CONCLUSIONS: The dose level 27 mg/kg of Makaradhwaja was found as NOAEL and dose level 13.5 mg/kg of Makaradhwaja was found as NOEL.
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spelling pubmed-61539182018-09-25 Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats Jamadagni, Shrirang Jamadagni, Pallavi Shrirang Singh, Rajendra Kumar Upadhyay, Sachchidanand Gaidhani, Sudesh N. Hazra, Jayram Ayu Pharmacological Study CONTEXT: Makaradhwaja is a Kupipakwa Rasayana. Since it contains two heavy metals, namely mercury and gold, it is essential to evaluate its safety. Hence, the present study was undertaken with an objective to evaluate toxicity and target organ of toxicity of Makaradhwaja if so. AIMS: The objective was to evaluate toxicological profile, the target organ of toxicity and to find no observed effect level (NOEL) or no observed adverse effect level (NOAEL) in rats after oral administration for ninety consecutive days. MATERIALS AND METHODS: Makaradhawaja preparation was administered to male and female Wistar rats for ninety consecutive days at 2.7, 13.5, and 27 mg/kg body weight. All relevant biochemical and hematological changes were observed. At termination, all the rats were sacrificed and necropsy was performed. Histopathological evaluation was also performed. STATISTICAL ANALYSIS USED: Dunnett's test followed by analysis of variance. RESULTS: There was a significant increase in high-dose group kidney weight of both sexes which could not be correlated with histopathology findings and serum biochemistry. Therefore, the change was not considered as an adverse effect. CONCLUSIONS: The dose level 27 mg/kg of Makaradhwaja was found as NOAEL and dose level 13.5 mg/kg of Makaradhwaja was found as NOEL. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC6153918/ /pubmed/30254400 http://dx.doi.org/10.4103/ayu.AYU_33_17 Text en Copyright: © 2018 AYU (An international quarterly journal of research in Ayurveda) http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Pharmacological Study
Jamadagni, Shrirang
Jamadagni, Pallavi Shrirang
Singh, Rajendra Kumar
Upadhyay, Sachchidanand
Gaidhani, Sudesh N.
Hazra, Jayram
Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats
title Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats
title_full Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats
title_fullStr Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats
title_full_unstemmed Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats
title_short Ninety days repeated dose oral toxicity study of Makaradhwaja in Wistar rats
title_sort ninety days repeated dose oral toxicity study of makaradhwaja in wistar rats
topic Pharmacological Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153918/
https://www.ncbi.nlm.nih.gov/pubmed/30254400
http://dx.doi.org/10.4103/ayu.AYU_33_17
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