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Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare
Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154099/ https://www.ncbi.nlm.nih.gov/pubmed/28397764 http://dx.doi.org/10.3390/molecules22040602 |
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author | Al Akeel, Raid Mateen, Ayesha Syed, Rabbani Alyousef, Abdullah A. Shaik, Mohammed Rafi |
author_facet | Al Akeel, Raid Mateen, Ayesha Syed, Rabbani Alyousef, Abdullah A. Shaik, Mohammed Rafi |
author_sort | Al Akeel, Raid |
collection | PubMed |
description | Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC(50) value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins. |
format | Online Article Text |
id | pubmed-6154099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61540992018-11-13 Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare Al Akeel, Raid Mateen, Ayesha Syed, Rabbani Alyousef, Abdullah A. Shaik, Mohammed Rafi Molecules Article Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC(50) value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins. MDPI 2017-04-08 /pmc/articles/PMC6154099/ /pubmed/28397764 http://dx.doi.org/10.3390/molecules22040602 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Al Akeel, Raid Mateen, Ayesha Syed, Rabbani Alyousef, Abdullah A. Shaik, Mohammed Rafi Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare |
title | Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare |
title_full | Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare |
title_fullStr | Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare |
title_full_unstemmed | Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare |
title_short | Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare |
title_sort | screening, purification and characterization of anionic antimicrobial proteins from foeniculum vulgare |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154099/ https://www.ncbi.nlm.nih.gov/pubmed/28397764 http://dx.doi.org/10.3390/molecules22040602 |
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