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Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines

Caesalpinia coriaria (C. coriaria), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on Caesalpinia species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells...

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Autores principales: Sánchez-Carranza, Jessica Nayelli, Alvarez, Laura, Marquina-Bahena, Silvia, Salas-Vidal, Enrique, Cuevas, Verónica, Jiménez, Elizabeth W., Veloz G., Rafael A., Carraz, Maelle, González-Maya, Leticia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154320/
https://www.ncbi.nlm.nih.gov/pubmed/28441723
http://dx.doi.org/10.3390/molecules22040666
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author Sánchez-Carranza, Jessica Nayelli
Alvarez, Laura
Marquina-Bahena, Silvia
Salas-Vidal, Enrique
Cuevas, Verónica
Jiménez, Elizabeth W.
Veloz G., Rafael A.
Carraz, Maelle
González-Maya, Leticia
author_facet Sánchez-Carranza, Jessica Nayelli
Alvarez, Laura
Marquina-Bahena, Silvia
Salas-Vidal, Enrique
Cuevas, Verónica
Jiménez, Elizabeth W.
Veloz G., Rafael A.
Carraz, Maelle
González-Maya, Leticia
author_sort Sánchez-Carranza, Jessica Nayelli
collection PubMed
description Caesalpinia coriaria (C. coriaria), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on Caesalpinia species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells. The aim of this study was to isolate and identify the active compounds of a water:acetone:ethanol (WAE) extract of C. coriaria pods and characterize their cytotoxic effect and cell death induction in different cancer cell lines. The compounds isolated and identified by chromatography and spectroscopic analysis were stigmasterol, ethyl gallate and gallic acid. Cytotoxic assays on cancer cells showed different ranges of activities. A differential effect on cell cycle progression was observed by flow cytometry. In particular, ethyl gallate and tannic acid induced G2/M phase cell cycle arrest and showed interesting effect on microtubule stabilization in Hep3B cells observed by immunofluorescence. The induction of apoptosis was characterized by morphological characteristic changes, and was supported by increases in the ratio of Bax/Bcl-2 expression and activation of caspase 3/7. This work constitutes the first phytochemical and cytotoxic study of C. coriaria and showed the action of its phenolic constituents on cell cycle, cell death and microtubules organization.
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spelling pubmed-61543202018-11-13 Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines Sánchez-Carranza, Jessica Nayelli Alvarez, Laura Marquina-Bahena, Silvia Salas-Vidal, Enrique Cuevas, Verónica Jiménez, Elizabeth W. Veloz G., Rafael A. Carraz, Maelle González-Maya, Leticia Molecules Article Caesalpinia coriaria (C. coriaria), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on Caesalpinia species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells. The aim of this study was to isolate and identify the active compounds of a water:acetone:ethanol (WAE) extract of C. coriaria pods and characterize their cytotoxic effect and cell death induction in different cancer cell lines. The compounds isolated and identified by chromatography and spectroscopic analysis were stigmasterol, ethyl gallate and gallic acid. Cytotoxic assays on cancer cells showed different ranges of activities. A differential effect on cell cycle progression was observed by flow cytometry. In particular, ethyl gallate and tannic acid induced G2/M phase cell cycle arrest and showed interesting effect on microtubule stabilization in Hep3B cells observed by immunofluorescence. The induction of apoptosis was characterized by morphological characteristic changes, and was supported by increases in the ratio of Bax/Bcl-2 expression and activation of caspase 3/7. This work constitutes the first phytochemical and cytotoxic study of C. coriaria and showed the action of its phenolic constituents on cell cycle, cell death and microtubules organization. MDPI 2017-04-22 /pmc/articles/PMC6154320/ /pubmed/28441723 http://dx.doi.org/10.3390/molecules22040666 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Carranza, Jessica Nayelli
Alvarez, Laura
Marquina-Bahena, Silvia
Salas-Vidal, Enrique
Cuevas, Verónica
Jiménez, Elizabeth W.
Veloz G., Rafael A.
Carraz, Maelle
González-Maya, Leticia
Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
title Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
title_full Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
title_fullStr Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
title_full_unstemmed Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
title_short Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
title_sort phenolic compounds isolated from caesalpinia coriaria induce s and g2/m phase cell cycle arrest differentially and trigger cell death by interfering with microtubule dynamics in cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154320/
https://www.ncbi.nlm.nih.gov/pubmed/28441723
http://dx.doi.org/10.3390/molecules22040666
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