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Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways

Gubenyiliu II (GYII), a Traditional Chinese Medicine (TCM) formula used in our hospital, has shown beneficial effects in cancer patients. In this study, we investigated the molecular mechanisms underlying the beneficial effects of GYII on murine breast cancer models. GYII showed significant inhibito...

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Autores principales: Zhang, Yi, Zhang, Gan-Lin, Sun, Xu, Cao, Ke-Xin, Shang, Ya-Wen, Gong, Mu-Xin, Ma, Cong, Nan, Nan, Li, Jin-Ping, Yu, Ming-Wei, Yang, Guo-Wang, Wang, Xiao-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154566/
https://www.ncbi.nlm.nih.gov/pubmed/28505136
http://dx.doi.org/10.3390/molecules22050787
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author Zhang, Yi
Zhang, Gan-Lin
Sun, Xu
Cao, Ke-Xin
Shang, Ya-Wen
Gong, Mu-Xin
Ma, Cong
Nan, Nan
Li, Jin-Ping
Yu, Ming-Wei
Yang, Guo-Wang
Wang, Xiao-Min
author_facet Zhang, Yi
Zhang, Gan-Lin
Sun, Xu
Cao, Ke-Xin
Shang, Ya-Wen
Gong, Mu-Xin
Ma, Cong
Nan, Nan
Li, Jin-Ping
Yu, Ming-Wei
Yang, Guo-Wang
Wang, Xiao-Min
author_sort Zhang, Yi
collection PubMed
description Gubenyiliu II (GYII), a Traditional Chinese Medicine (TCM) formula used in our hospital, has shown beneficial effects in cancer patients. In this study, we investigated the molecular mechanisms underlying the beneficial effects of GYII on murine breast cancer models. GYII showed significant inhibitory effects on tumor growth and metastasis in the murine breast cancer model. Additionally, GYII suppressed the proliferation of 4T1 and MCF-7 cells in a dose-dependent manner. A better inhibitory effect on 4T1 cell proliferation and migration was found in the decomposed recipes (DR) of GYII. Moreover, heparanase expression and the degree of angiogenesis were reduced in tumor tissues. Western blot analysis showed decreased expression of heparanase and growth factors in the cells treated with GYII and its decomposed recipes (DR2 and DR3), and thereby a reduction in the phosphorylation of extracellular signal-regulated kinase (ERK) and serine-threonine kinase (AKT). These results suggest that GYII exerts anti-tumor growth and anti-metastatic effects in the murine breast cancer model. The anti-tumor activity of GYII and its decomposed recipes is, at least partly, associated with decreased heparanase and growth factor expression, which subsequently suppressed the activation of the ERK and AKT pathways.
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spelling pubmed-61545662018-11-13 Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways Zhang, Yi Zhang, Gan-Lin Sun, Xu Cao, Ke-Xin Shang, Ya-Wen Gong, Mu-Xin Ma, Cong Nan, Nan Li, Jin-Ping Yu, Ming-Wei Yang, Guo-Wang Wang, Xiao-Min Molecules Article Gubenyiliu II (GYII), a Traditional Chinese Medicine (TCM) formula used in our hospital, has shown beneficial effects in cancer patients. In this study, we investigated the molecular mechanisms underlying the beneficial effects of GYII on murine breast cancer models. GYII showed significant inhibitory effects on tumor growth and metastasis in the murine breast cancer model. Additionally, GYII suppressed the proliferation of 4T1 and MCF-7 cells in a dose-dependent manner. A better inhibitory effect on 4T1 cell proliferation and migration was found in the decomposed recipes (DR) of GYII. Moreover, heparanase expression and the degree of angiogenesis were reduced in tumor tissues. Western blot analysis showed decreased expression of heparanase and growth factors in the cells treated with GYII and its decomposed recipes (DR2 and DR3), and thereby a reduction in the phosphorylation of extracellular signal-regulated kinase (ERK) and serine-threonine kinase (AKT). These results suggest that GYII exerts anti-tumor growth and anti-metastatic effects in the murine breast cancer model. The anti-tumor activity of GYII and its decomposed recipes is, at least partly, associated with decreased heparanase and growth factor expression, which subsequently suppressed the activation of the ERK and AKT pathways. MDPI 2017-05-15 /pmc/articles/PMC6154566/ /pubmed/28505136 http://dx.doi.org/10.3390/molecules22050787 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yi
Zhang, Gan-Lin
Sun, Xu
Cao, Ke-Xin
Shang, Ya-Wen
Gong, Mu-Xin
Ma, Cong
Nan, Nan
Li, Jin-Ping
Yu, Ming-Wei
Yang, Guo-Wang
Wang, Xiao-Min
Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
title Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
title_full Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
title_fullStr Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
title_full_unstemmed Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
title_short Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
title_sort gubenyiliu ii inhibits breast tumor growth and metastasis associated with decreased heparanase expression and phosphorylation of erk and akt pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154566/
https://www.ncbi.nlm.nih.gov/pubmed/28505136
http://dx.doi.org/10.3390/molecules22050787
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