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Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor Platform
In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. Of the 21 aptamers tes...
Autores principales: | Song, Myeong-Sub, Sekhon, Simranjeet Singh, Shin, Woo-Ri, Kim, Hyung Cheol, Min, Jiho, Ahn, Ji-Young, Kim, Yang-Hoon |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154610/ https://www.ncbi.nlm.nih.gov/pubmed/28513559 http://dx.doi.org/10.3390/molecules22050825 |
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