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In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium
Two new ubiquinones, named antrocinnamone and 4-acetylantrocamol LT3, were isolated along with six known ubiquinones from Antrodia cinnamomea (Polyporaceae) mycelium. The developed HPLC analysis methods successfully identified eight different ubiquinones, two benzenoids, and one maleic acid derivati...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154633/ https://www.ncbi.nlm.nih.gov/pubmed/28481255 http://dx.doi.org/10.3390/molecules22050747 |
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author | Yen, I-Chuan Lee, Shih-Yu Lin, Kuen-Tze Lai, Feng-Yi Kuo, Mao-Tien Chang, Wen-Liang |
author_facet | Yen, I-Chuan Lee, Shih-Yu Lin, Kuen-Tze Lai, Feng-Yi Kuo, Mao-Tien Chang, Wen-Liang |
author_sort | Yen, I-Chuan |
collection | PubMed |
description | Two new ubiquinones, named antrocinnamone and 4-acetylantrocamol LT3, were isolated along with six known ubiquinones from Antrodia cinnamomea (Polyporaceae) mycelium. The developed HPLC analysis methods successfully identified eight different ubiquinones, two benzenoids, and one maleic acid derivative from A. cinnamomea. The ubiquinones 1–8 exhibited potential and selective cytotoxic activity against three human cancer cell lines, with IC(50) values ranging from 0.001 to 35.883 μM. We suggest that the different cytotoxicity levels were related to their chemical structures, especially the 4-hydroxycyclohex-2-enone ring and the presence of a free hydroxyl group in the side chain. The suppression by 4-acetylantrocamol LT3 stopped the cell cycle at the beginning of the G2-M phase thus making the cell cycle arrest at the sub-G1 phase as compared with control cells. |
format | Online Article Text |
id | pubmed-6154633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61546332018-11-13 In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium Yen, I-Chuan Lee, Shih-Yu Lin, Kuen-Tze Lai, Feng-Yi Kuo, Mao-Tien Chang, Wen-Liang Molecules Article Two new ubiquinones, named antrocinnamone and 4-acetylantrocamol LT3, were isolated along with six known ubiquinones from Antrodia cinnamomea (Polyporaceae) mycelium. The developed HPLC analysis methods successfully identified eight different ubiquinones, two benzenoids, and one maleic acid derivative from A. cinnamomea. The ubiquinones 1–8 exhibited potential and selective cytotoxic activity against three human cancer cell lines, with IC(50) values ranging from 0.001 to 35.883 μM. We suggest that the different cytotoxicity levels were related to their chemical structures, especially the 4-hydroxycyclohex-2-enone ring and the presence of a free hydroxyl group in the side chain. The suppression by 4-acetylantrocamol LT3 stopped the cell cycle at the beginning of the G2-M phase thus making the cell cycle arrest at the sub-G1 phase as compared with control cells. MDPI 2017-05-06 /pmc/articles/PMC6154633/ /pubmed/28481255 http://dx.doi.org/10.3390/molecules22050747 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yen, I-Chuan Lee, Shih-Yu Lin, Kuen-Tze Lai, Feng-Yi Kuo, Mao-Tien Chang, Wen-Liang In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium |
title | In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium |
title_full | In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium |
title_fullStr | In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium |
title_full_unstemmed | In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium |
title_short | In Vitro Anticancer Activity and Structural Characterization of Ubiquinones from Antrodia cinnamomea Mycelium |
title_sort | in vitro anticancer activity and structural characterization of ubiquinones from antrodia cinnamomea mycelium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154633/ https://www.ncbi.nlm.nih.gov/pubmed/28481255 http://dx.doi.org/10.3390/molecules22050747 |
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