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Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa

Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. Turnera diffusa (damiana) is a shrub that grows throughout Mexico and is traditionally used for many illnesses including diabetes. Although a large number of plant...

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Autores principales: Parra-Naranjo, Aída, Delgado-Montemayor, Cecilia, Fraga-López, Alejandra, Castañeda-Corral, Gabriela, Salazar-Aranda, Ricardo, Acevedo-Fernández, Juan José, Waksman, Noemi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154680/
https://www.ncbi.nlm.nih.gov/pubmed/28397755
http://dx.doi.org/10.3390/molecules22040599
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author Parra-Naranjo, Aída
Delgado-Montemayor, Cecilia
Fraga-López, Alejandra
Castañeda-Corral, Gabriela
Salazar-Aranda, Ricardo
Acevedo-Fernández, Juan José
Waksman, Noemi
author_facet Parra-Naranjo, Aída
Delgado-Montemayor, Cecilia
Fraga-López, Alejandra
Castañeda-Corral, Gabriela
Salazar-Aranda, Ricardo
Acevedo-Fernández, Juan José
Waksman, Noemi
author_sort Parra-Naranjo, Aída
collection PubMed
description Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. Turnera diffusa (damiana) is a shrub that grows throughout Mexico and is traditionally used for many illnesses including diabetes. Although a large number of plant metabolites are known, there are no reports indicating which of these are responsible for this activity, and this identification was the objective of the present work. Through bioassay-guided fractionation of a methanolic extract obtained from the aerial part of T. diffusa, teuhetenone A was isolated and identified as the main metabolite responsible for the plant’s hypoglycemic activity. Alpha-glucosidase inhibitory activity and cytotoxicity of this metabolite were determined. Hypoglycemic and antidiabetic activities were evaluated in a murine model of diabetes in vivo, by monitoring glucose levels for six hours and comparing them with levels after administering various controls. Teuhetenone A was not cytotoxic at the tested concentrations, and did not show inhibitory activity in the glucosidase test, and the in vivo assays showed a gradual reduction in glucose levels in normoglycemic and diabetic mice. Considering these results, we suggest that teuhetenone A has potential as an antidiabetic compound, which could be further submitted to preclinical assays.
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spelling pubmed-61546802018-11-13 Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa Parra-Naranjo, Aída Delgado-Montemayor, Cecilia Fraga-López, Alejandra Castañeda-Corral, Gabriela Salazar-Aranda, Ricardo Acevedo-Fernández, Juan José Waksman, Noemi Molecules Article Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. Turnera diffusa (damiana) is a shrub that grows throughout Mexico and is traditionally used for many illnesses including diabetes. Although a large number of plant metabolites are known, there are no reports indicating which of these are responsible for this activity, and this identification was the objective of the present work. Through bioassay-guided fractionation of a methanolic extract obtained from the aerial part of T. diffusa, teuhetenone A was isolated and identified as the main metabolite responsible for the plant’s hypoglycemic activity. Alpha-glucosidase inhibitory activity and cytotoxicity of this metabolite were determined. Hypoglycemic and antidiabetic activities were evaluated in a murine model of diabetes in vivo, by monitoring glucose levels for six hours and comparing them with levels after administering various controls. Teuhetenone A was not cytotoxic at the tested concentrations, and did not show inhibitory activity in the glucosidase test, and the in vivo assays showed a gradual reduction in glucose levels in normoglycemic and diabetic mice. Considering these results, we suggest that teuhetenone A has potential as an antidiabetic compound, which could be further submitted to preclinical assays. MDPI 2017-04-08 /pmc/articles/PMC6154680/ /pubmed/28397755 http://dx.doi.org/10.3390/molecules22040599 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parra-Naranjo, Aída
Delgado-Montemayor, Cecilia
Fraga-López, Alejandra
Castañeda-Corral, Gabriela
Salazar-Aranda, Ricardo
Acevedo-Fernández, Juan José
Waksman, Noemi
Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa
title Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa
title_full Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa
title_fullStr Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa
title_full_unstemmed Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa
title_short Acute Hypoglycemic and Antidiabetic Effect of Teuhetenone A Isolated from Turnera diffusa
title_sort acute hypoglycemic and antidiabetic effect of teuhetenone a isolated from turnera diffusa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154680/
https://www.ncbi.nlm.nih.gov/pubmed/28397755
http://dx.doi.org/10.3390/molecules22040599
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