Cargando…

Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species

Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in...

Descripción completa

Detalles Bibliográficos
Autores principales: Bado, Inés, Papa-Ezdra, Romina, Delgado-Blas, Jose F., Gaudio, Micaela, Gutiérrez, Claudia, Cordeiro, Nicolás F., García-Fulgueiras, Virginia, Araújo Pirez, Lucía, Seija, Verónica, Medina, Julio C., Rieppi, Gloria, Gonzalez-Zorn, Bruno, Vignoli, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154759/
https://www.ncbi.nlm.nih.gov/pubmed/29920143
http://dx.doi.org/10.1089/mdr.2017.0300
_version_ 1783357758379130880
author Bado, Inés
Papa-Ezdra, Romina
Delgado-Blas, Jose F.
Gaudio, Micaela
Gutiérrez, Claudia
Cordeiro, Nicolás F.
García-Fulgueiras, Virginia
Araújo Pirez, Lucía
Seija, Verónica
Medina, Julio C.
Rieppi, Gloria
Gonzalez-Zorn, Bruno
Vignoli, Rafael
author_facet Bado, Inés
Papa-Ezdra, Romina
Delgado-Blas, Jose F.
Gaudio, Micaela
Gutiérrez, Claudia
Cordeiro, Nicolás F.
García-Fulgueiras, Virginia
Araújo Pirez, Lucía
Seija, Verónica
Medina, Julio C.
Rieppi, Gloria
Gonzalez-Zorn, Bruno
Vignoli, Rafael
author_sort Bado, Inés
collection PubMed
description Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however, no information exists in this region regarding the occurrence of 16S-RMTases in this microorganism. In addition, the epidemiology of A. baumannii in ICUs and its associated resistance profiles are poorly understood. Our objectives were as follows: to study the clonal relationship and antibiotic resistance profiles of clinical and digestive colonizing A. baumannii isolates in an ICU, to characterize the circulating carbapenemases, and to detect 16S-RMTases. Patients admitted between August 2010 and July 2011 with a clinically predicted hospital stay > 48 hr were included. Pharyngeal and rectal swabs were obtained during the first fortnight after hospitalization. Resistance profiles were determined with MicroScan(®) and VITEK2 system. Carbapenemases and 16S-RMTases were identified by PCR and sequencing, and clonality was assessed by pulsed-field gel electrophoresis and multilocus sequence typing. Sixty-nine patients were studied and 63 were diagnosed with bacterial infections. Among these, 29 were CRAB isolates; 49 A. baumannii were isolated as digestive colonizers. These 78 isolates were clustered in 7 pulsetypes, mostly belonging to ST79. The only carbapenemase genes detected were bla(OXA-51) (n = 78), bla(OXA-23) (n = 62), and bla(OXA-58) (n = 3). Interestingly, two clinical isolates harbored the rmtC 16S-RMTase gene. To the best of our knowledge, this is the first description of the presence of rmtC in A. baumannii.
format Online
Article
Text
id pubmed-6154759
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Mary Ann Liebert, Inc., publishers
record_format MEDLINE/PubMed
spelling pubmed-61547592018-10-03 Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species Bado, Inés Papa-Ezdra, Romina Delgado-Blas, Jose F. Gaudio, Micaela Gutiérrez, Claudia Cordeiro, Nicolás F. García-Fulgueiras, Virginia Araújo Pirez, Lucía Seija, Verónica Medina, Julio C. Rieppi, Gloria Gonzalez-Zorn, Bruno Vignoli, Rafael Microb Drug Resist Epidemiology Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however, no information exists in this region regarding the occurrence of 16S-RMTases in this microorganism. In addition, the epidemiology of A. baumannii in ICUs and its associated resistance profiles are poorly understood. Our objectives were as follows: to study the clonal relationship and antibiotic resistance profiles of clinical and digestive colonizing A. baumannii isolates in an ICU, to characterize the circulating carbapenemases, and to detect 16S-RMTases. Patients admitted between August 2010 and July 2011 with a clinically predicted hospital stay > 48 hr were included. Pharyngeal and rectal swabs were obtained during the first fortnight after hospitalization. Resistance profiles were determined with MicroScan(®) and VITEK2 system. Carbapenemases and 16S-RMTases were identified by PCR and sequencing, and clonality was assessed by pulsed-field gel electrophoresis and multilocus sequence typing. Sixty-nine patients were studied and 63 were diagnosed with bacterial infections. Among these, 29 were CRAB isolates; 49 A. baumannii were isolated as digestive colonizers. These 78 isolates were clustered in 7 pulsetypes, mostly belonging to ST79. The only carbapenemase genes detected were bla(OXA-51) (n = 78), bla(OXA-23) (n = 62), and bla(OXA-58) (n = 3). Interestingly, two clinical isolates harbored the rmtC 16S-RMTase gene. To the best of our knowledge, this is the first description of the presence of rmtC in A. baumannii. Mary Ann Liebert, Inc., publishers 2018-09-01 2018-09-10 /pmc/articles/PMC6154759/ /pubmed/29920143 http://dx.doi.org/10.1089/mdr.2017.0300 Text en © Inés Bado et al., 2018; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.
spellingShingle Epidemiology
Bado, Inés
Papa-Ezdra, Romina
Delgado-Blas, Jose F.
Gaudio, Micaela
Gutiérrez, Claudia
Cordeiro, Nicolás F.
García-Fulgueiras, Virginia
Araújo Pirez, Lucía
Seija, Verónica
Medina, Julio C.
Rieppi, Gloria
Gonzalez-Zorn, Bruno
Vignoli, Rafael
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species
title Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species
title_full Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species
title_fullStr Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species
title_full_unstemmed Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species
title_short Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay's University Hospital Identifies the First rmtC Gene in the Species
title_sort molecular characterization of carbapenem-resistant acinetobacter baumannii in the intensive care unit of uruguay's university hospital identifies the first rmtc gene in the species
topic Epidemiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154759/
https://www.ncbi.nlm.nih.gov/pubmed/29920143
http://dx.doi.org/10.1089/mdr.2017.0300
work_keys_str_mv AT badoines molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT papaezdraromina molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT delgadoblasjosef molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT gaudiomicaela molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT gutierrezclaudia molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT cordeironicolasf molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT garciafulgueirasvirginia molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT araujopirezlucia molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT seijaveronica molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT medinajulioc molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT rieppigloria molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT gonzalezzornbruno molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies
AT vignolirafael molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniiintheintensivecareunitofuruguaysuniversityhospitalidentifiesthefirstrmtcgeneinthespecies