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The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation

Chromatin remodelers regulate the nucleosome barrier during transcription, DNA replication, and DNA repair. The chromatin remodeler RSF1 is enriched at mitotic centromeres, but the functional consequences of this enrichment are not completely understood. Shugoshin (Sgo1) protects centromeric cohesio...

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Autores principales: Lee, Ho-Soo, Lin, Zhonghui, Chae, Sunyoung, Yoo, Young-Suk, Kim, Byung-Gyu, Lee, Youngsoo, Johnson, Jared L., Kim, You-Sun, Cantley, Lewis C., Lee, Chang-Woo, Yu, Hongtao, Cho, Hyeseong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155007/
https://www.ncbi.nlm.nih.gov/pubmed/30242288
http://dx.doi.org/10.1038/s41467-018-06377-w
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author Lee, Ho-Soo
Lin, Zhonghui
Chae, Sunyoung
Yoo, Young-Suk
Kim, Byung-Gyu
Lee, Youngsoo
Johnson, Jared L.
Kim, You-Sun
Cantley, Lewis C.
Lee, Chang-Woo
Yu, Hongtao
Cho, Hyeseong
author_facet Lee, Ho-Soo
Lin, Zhonghui
Chae, Sunyoung
Yoo, Young-Suk
Kim, Byung-Gyu
Lee, Youngsoo
Johnson, Jared L.
Kim, You-Sun
Cantley, Lewis C.
Lee, Chang-Woo
Yu, Hongtao
Cho, Hyeseong
author_sort Lee, Ho-Soo
collection PubMed
description Chromatin remodelers regulate the nucleosome barrier during transcription, DNA replication, and DNA repair. The chromatin remodeler RSF1 is enriched at mitotic centromeres, but the functional consequences of this enrichment are not completely understood. Shugoshin (Sgo1) protects centromeric cohesion during mitosis and requires BuB1-dependent histone H2A phosphorylation (H2A-pT120) for localization. Loss of Sgo1 at centromeres causes chromosome missegregation. Here, we show that RSF1 regulates Sgo1 localization to centromeres through coordinating a crosstalk between histone acetylation and phosphorylation. RSF1 interacts with and recruits HDAC1 to centromeres, where it counteracts TIP60-mediated acetylation of H2A at K118. This deacetylation is required for the accumulation of H2A-pT120 and Sgo1 deposition, as H2A-K118 acetylation suppresses H2A-T120 phosphorylation by Bub1. Centromeric tethering of HDAC1 prevents premature chromatid separation in RSF1 knockout cells. Our results indicate that RSF1 regulates the dynamics of H2A histone modifications at mitotic centromeres and contributes to the maintenance of chromosome stability.
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spelling pubmed-61550072018-09-28 The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation Lee, Ho-Soo Lin, Zhonghui Chae, Sunyoung Yoo, Young-Suk Kim, Byung-Gyu Lee, Youngsoo Johnson, Jared L. Kim, You-Sun Cantley, Lewis C. Lee, Chang-Woo Yu, Hongtao Cho, Hyeseong Nat Commun Article Chromatin remodelers regulate the nucleosome barrier during transcription, DNA replication, and DNA repair. The chromatin remodeler RSF1 is enriched at mitotic centromeres, but the functional consequences of this enrichment are not completely understood. Shugoshin (Sgo1) protects centromeric cohesion during mitosis and requires BuB1-dependent histone H2A phosphorylation (H2A-pT120) for localization. Loss of Sgo1 at centromeres causes chromosome missegregation. Here, we show that RSF1 regulates Sgo1 localization to centromeres through coordinating a crosstalk between histone acetylation and phosphorylation. RSF1 interacts with and recruits HDAC1 to centromeres, where it counteracts TIP60-mediated acetylation of H2A at K118. This deacetylation is required for the accumulation of H2A-pT120 and Sgo1 deposition, as H2A-K118 acetylation suppresses H2A-T120 phosphorylation by Bub1. Centromeric tethering of HDAC1 prevents premature chromatid separation in RSF1 knockout cells. Our results indicate that RSF1 regulates the dynamics of H2A histone modifications at mitotic centromeres and contributes to the maintenance of chromosome stability. Nature Publishing Group UK 2018-09-21 /pmc/articles/PMC6155007/ /pubmed/30242288 http://dx.doi.org/10.1038/s41467-018-06377-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lee, Ho-Soo
Lin, Zhonghui
Chae, Sunyoung
Yoo, Young-Suk
Kim, Byung-Gyu
Lee, Youngsoo
Johnson, Jared L.
Kim, You-Sun
Cantley, Lewis C.
Lee, Chang-Woo
Yu, Hongtao
Cho, Hyeseong
The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation
title The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation
title_full The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation
title_fullStr The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation
title_full_unstemmed The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation
title_short The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation
title_sort chromatin remodeler rsf1 controls centromeric histone modifications to coordinate chromosome segregation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155007/
https://www.ncbi.nlm.nih.gov/pubmed/30242288
http://dx.doi.org/10.1038/s41467-018-06377-w
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