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Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway
Paeoniflorin (PF) is an active ingredient of Paeoniae Radix which possesses the neuroprotective effect. However, so far, the neuroprotective mechanism of PF has still not been fully uncovered. The Ca(2+)/Ca(2+)/calmodulin-dependent protein kinase II (CaMKII)/cAMP response element-binding (CREB) sign...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155252/ https://www.ncbi.nlm.nih.gov/pubmed/28264448 http://dx.doi.org/10.3390/molecules22030359 |
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author | Zhang, Yuqin Qiao, Lifei Xu, Wen Wang, Xiaoying Li, Huang Xu, Wei Chu, Kedan Lin, Yu |
author_facet | Zhang, Yuqin Qiao, Lifei Xu, Wen Wang, Xiaoying Li, Huang Xu, Wei Chu, Kedan Lin, Yu |
author_sort | Zhang, Yuqin |
collection | PubMed |
description | Paeoniflorin (PF) is an active ingredient of Paeoniae Radix which possesses the neuroprotective effect. However, so far, the neuroprotective mechanism of PF has still not been fully uncovered. The Ca(2+)/Ca(2+)/calmodulin-dependent protein kinase II (CaMKII)/cAMP response element-binding (CREB) signaling pathway plays an important role in the intracellular signal transduction pathway involved in cell proliferation, cell survival, inflammation and metabolism. Herein, the neuroprotective roles of PF in the models of middle cerebral artery occlusion (MCAO) followed by reperfusion in rats and N-methyl-d-aspartic acid (NMDA)-induced excitotoxicity in primary hippocampal neurons were investigated. Moreover, we attempted to confirm the hypothesis that its protection effect is via the modulation of the Ca(2+)/CaMKI)/CREB signaling pathway. In this study, PF not only significantly decreased neurological deficit scores and infarct volume in vivo, but also improved neurons’ cell viability, and inhibited neurons’ apoptosis and intracellular Ca(2+) concentration in vitro. Furthermore, PF significantly up-regulated p-CREB and p-CaMKII, and down-regulated calmodulin (CaM) in vivo and in vitro. The results indicate that the protective effect of PF on cerebral ischemia reperfusion injury is possible through regulating the Ca(2+)/CaMKII/CREB signaling pathway. |
format | Online Article Text |
id | pubmed-6155252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61552522018-11-13 Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway Zhang, Yuqin Qiao, Lifei Xu, Wen Wang, Xiaoying Li, Huang Xu, Wei Chu, Kedan Lin, Yu Molecules Article Paeoniflorin (PF) is an active ingredient of Paeoniae Radix which possesses the neuroprotective effect. However, so far, the neuroprotective mechanism of PF has still not been fully uncovered. The Ca(2+)/Ca(2+)/calmodulin-dependent protein kinase II (CaMKII)/cAMP response element-binding (CREB) signaling pathway plays an important role in the intracellular signal transduction pathway involved in cell proliferation, cell survival, inflammation and metabolism. Herein, the neuroprotective roles of PF in the models of middle cerebral artery occlusion (MCAO) followed by reperfusion in rats and N-methyl-d-aspartic acid (NMDA)-induced excitotoxicity in primary hippocampal neurons were investigated. Moreover, we attempted to confirm the hypothesis that its protection effect is via the modulation of the Ca(2+)/CaMKI)/CREB signaling pathway. In this study, PF not only significantly decreased neurological deficit scores and infarct volume in vivo, but also improved neurons’ cell viability, and inhibited neurons’ apoptosis and intracellular Ca(2+) concentration in vitro. Furthermore, PF significantly up-regulated p-CREB and p-CaMKII, and down-regulated calmodulin (CaM) in vivo and in vitro. The results indicate that the protective effect of PF on cerebral ischemia reperfusion injury is possible through regulating the Ca(2+)/CaMKII/CREB signaling pathway. MDPI 2017-02-27 /pmc/articles/PMC6155252/ /pubmed/28264448 http://dx.doi.org/10.3390/molecules22030359 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Yuqin Qiao, Lifei Xu, Wen Wang, Xiaoying Li, Huang Xu, Wei Chu, Kedan Lin, Yu Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway |
title | Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway |
title_full | Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway |
title_fullStr | Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway |
title_full_unstemmed | Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway |
title_short | Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca(2+)/CaMKII/CREB Signaling Pathway |
title_sort | paeoniflorin attenuates cerebral ischemia-induced injury by regulating ca(2+)/camkii/creb signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155252/ https://www.ncbi.nlm.nih.gov/pubmed/28264448 http://dx.doi.org/10.3390/molecules22030359 |
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