Cargando…
Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts
We investigated the effects of natural plant hormones, indole-3-acetic (IAA) acid and gibberellic acid (GA), on the growth parameters and production of flavonoids and other phenolic compounds in common buckwheat sprouts. A total of 17 phenolic compounds were identified using liquid chromatography-ma...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155264/ https://www.ncbi.nlm.nih.gov/pubmed/28264513 http://dx.doi.org/10.3390/molecules22030374 |
_version_ | 1783357862714540032 |
---|---|
author | Park, Chang Ha Yeo, Hyeon Ji Park, Yun Ji Morgan, Abubaker M. A. Valan Arasu, Mariadhas Al-Dhabi, Naif Abdullah Park, Sang Un |
author_facet | Park, Chang Ha Yeo, Hyeon Ji Park, Yun Ji Morgan, Abubaker M. A. Valan Arasu, Mariadhas Al-Dhabi, Naif Abdullah Park, Sang Un |
author_sort | Park, Chang Ha |
collection | PubMed |
description | We investigated the effects of natural plant hormones, indole-3-acetic (IAA) acid and gibberellic acid (GA), on the growth parameters and production of flavonoids and other phenolic compounds in common buckwheat sprouts. A total of 17 phenolic compounds were identified using liquid chromatography-mass spectrometry (LC-MS) analysis. Among these, seven compounds (4-hydroxybenzoic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, and quercetin) were quantified by high-performance liquid chromatography (HPLC) after treating the common buckwheat sprouts with different concentrations of the hormones IAA and GA. At a concentration of 0.5 mg/L, both IAA and GA exhibited the highest levels of growth parameters (shoot length, root length, and fresh weight). The HPLC analysis showed that the treatment of sprouts with IAA at concentrations ranging from 0.1 to 1.0 mg/L produced higher or comparable levels of the total phenolic compounds than the control sprout and enhanced the production of rutin. Similarly, the supplementation with 0.1 and 0.5 mg/L GA increased the content of rutin in buckwheat sprouts. Our results suggested that the treatment with optimal concentrations of IAA and GA enhanced the growth parameters and accumulation of flavonoids and other phenolic compounds in buckwheat sprouts. |
format | Online Article Text |
id | pubmed-6155264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61552642018-11-13 Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts Park, Chang Ha Yeo, Hyeon Ji Park, Yun Ji Morgan, Abubaker M. A. Valan Arasu, Mariadhas Al-Dhabi, Naif Abdullah Park, Sang Un Molecules Article We investigated the effects of natural plant hormones, indole-3-acetic (IAA) acid and gibberellic acid (GA), on the growth parameters and production of flavonoids and other phenolic compounds in common buckwheat sprouts. A total of 17 phenolic compounds were identified using liquid chromatography-mass spectrometry (LC-MS) analysis. Among these, seven compounds (4-hydroxybenzoic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, and quercetin) were quantified by high-performance liquid chromatography (HPLC) after treating the common buckwheat sprouts with different concentrations of the hormones IAA and GA. At a concentration of 0.5 mg/L, both IAA and GA exhibited the highest levels of growth parameters (shoot length, root length, and fresh weight). The HPLC analysis showed that the treatment of sprouts with IAA at concentrations ranging from 0.1 to 1.0 mg/L produced higher or comparable levels of the total phenolic compounds than the control sprout and enhanced the production of rutin. Similarly, the supplementation with 0.1 and 0.5 mg/L GA increased the content of rutin in buckwheat sprouts. Our results suggested that the treatment with optimal concentrations of IAA and GA enhanced the growth parameters and accumulation of flavonoids and other phenolic compounds in buckwheat sprouts. MDPI 2017-02-28 /pmc/articles/PMC6155264/ /pubmed/28264513 http://dx.doi.org/10.3390/molecules22030374 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Chang Ha Yeo, Hyeon Ji Park, Yun Ji Morgan, Abubaker M. A. Valan Arasu, Mariadhas Al-Dhabi, Naif Abdullah Park, Sang Un Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts |
title | Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts |
title_full | Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts |
title_fullStr | Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts |
title_full_unstemmed | Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts |
title_short | Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts |
title_sort | influence of indole-3-acetic acid and gibberellic acid on phenylpropanoid accumulation in common buckwheat (fagopyrum esculentum moench) sprouts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155264/ https://www.ncbi.nlm.nih.gov/pubmed/28264513 http://dx.doi.org/10.3390/molecules22030374 |
work_keys_str_mv | AT parkchangha influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts AT yeohyeonji influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts AT parkyunji influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts AT morganabubakerma influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts AT valanarasumariadhas influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts AT aldhabinaifabdullah influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts AT parksangun influenceofindole3aceticacidandgibberellicacidonphenylpropanoidaccumulationincommonbuckwheatfagopyrumesculentummoenchsprouts |