Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite

Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO), a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE). Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS), which s...

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Detalles Bibliográficos
Autores principales: Wei, Zheng, Zhang, Junping, Zhang, Aihua, Wang, Yanchun, Cai, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155348/
https://www.ncbi.nlm.nih.gov/pubmed/28257099
http://dx.doi.org/10.3390/molecules22030392
Descripción
Sumario:Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO), a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE). Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS), which served as reducing and stabilizing agent. We showed that the sensitivity of the immunosensor designed in this report was remarkably enhanced because of the numerous active sites in the sensor provided by the AuNP-RGO nanostructure. For the quantification of NSE, the immunosensor exhibited a positive linear relationship with the concentration in the range of 0.1 to 2000 ng/mL, where the limit of the detection was 0.05 ng/mL.

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