Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite

Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO), a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE). Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS), which s...

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Detalles Bibliográficos
Autores principales: Wei, Zheng, Zhang, Junping, Zhang, Aihua, Wang, Yanchun, Cai, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155348/
https://www.ncbi.nlm.nih.gov/pubmed/28257099
http://dx.doi.org/10.3390/molecules22030392
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author Wei, Zheng
Zhang, Junping
Zhang, Aihua
Wang, Yanchun
Cai, Xiaoping
author_facet Wei, Zheng
Zhang, Junping
Zhang, Aihua
Wang, Yanchun
Cai, Xiaoping
author_sort Wei, Zheng
collection PubMed
description Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO), a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE). Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS), which served as reducing and stabilizing agent. We showed that the sensitivity of the immunosensor designed in this report was remarkably enhanced because of the numerous active sites in the sensor provided by the AuNP-RGO nanostructure. For the quantification of NSE, the immunosensor exhibited a positive linear relationship with the concentration in the range of 0.1 to 2000 ng/mL, where the limit of the detection was 0.05 ng/mL.
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spelling pubmed-61553482018-11-13 Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite Wei, Zheng Zhang, Junping Zhang, Aihua Wang, Yanchun Cai, Xiaoping Molecules Article Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO), a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE). Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS), which served as reducing and stabilizing agent. We showed that the sensitivity of the immunosensor designed in this report was remarkably enhanced because of the numerous active sites in the sensor provided by the AuNP-RGO nanostructure. For the quantification of NSE, the immunosensor exhibited a positive linear relationship with the concentration in the range of 0.1 to 2000 ng/mL, where the limit of the detection was 0.05 ng/mL. MDPI 2017-03-02 /pmc/articles/PMC6155348/ /pubmed/28257099 http://dx.doi.org/10.3390/molecules22030392 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Zheng
Zhang, Junping
Zhang, Aihua
Wang, Yanchun
Cai, Xiaoping
Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite
title Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite
title_full Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite
title_fullStr Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite
title_full_unstemmed Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite
title_short Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite
title_sort electrochemical detecting lung cancer-associated antigen based on graphene-gold nanocomposite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155348/
https://www.ncbi.nlm.nih.gov/pubmed/28257099
http://dx.doi.org/10.3390/molecules22030392
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AT zhangjunping electrochemicaldetectinglungcancerassociatedantigenbasedongraphenegoldnanocomposite
AT zhangaihua electrochemicaldetectinglungcancerassociatedantigenbasedongraphenegoldnanocomposite
AT wangyanchun electrochemicaldetectinglungcancerassociatedantigenbasedongraphenegoldnanocomposite
AT caixiaoping electrochemicaldetectinglungcancerassociatedantigenbasedongraphenegoldnanocomposite

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