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Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS

The ethnic drug Melastoma dodecandrum Lour. (MDL) is widely distributed throughout South China, and is the major component of Gong Yan Ping Tablets/Capsules and Zi Di Ning Xue San. Although the pharmacological effects of MDL have been well documented, its chemical profile has not been fully determin...

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Autores principales: Wang, Jinfeng, Jia, Ziyao, Zhang, Zhihao, Wang, Yutong, Liu, Xi, Wang, Linghua, Lin, Ruichao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155390/
https://www.ncbi.nlm.nih.gov/pubmed/28304342
http://dx.doi.org/10.3390/molecules22030476
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author Wang, Jinfeng
Jia, Ziyao
Zhang, Zhihao
Wang, Yutong
Liu, Xi
Wang, Linghua
Lin, Ruichao
author_facet Wang, Jinfeng
Jia, Ziyao
Zhang, Zhihao
Wang, Yutong
Liu, Xi
Wang, Linghua
Lin, Ruichao
author_sort Wang, Jinfeng
collection PubMed
description The ethnic drug Melastoma dodecandrum Lour. (MDL) is widely distributed throughout South China, and is the major component of Gong Yan Ping Tablets/Capsules and Zi Di Ning Xue San. Although the pharmacological effects of MDL have been well documented, its chemical profile has not been fully determined. In this study, we have developed a rapid and sensitive UPLC-ESI-Q-Exactive Focus-MS/MS method to characterize the chemical constituents of MDL in the positive and negative ionization modes. A comparison of the chromatographic and spectrometric data obtained using this method with data from databases, the literature and reference standards allowed us to identify or tentatively characterize 109 compounds, including 26 fatty acids, 26 organic acids, 33 flavonoids, six tannins, 10 triterpenoids, two steroids and six other compounds. Notably, 55 of the compounds characterized in this study have never been detected before in this plant. The information obtained in this study therefore enriches our understanding of the chemical composition of MDL and could be used in quality control, pharmacological research and the development of drugs based on MDL. In addition, this study represents the first reported comprehensive analysis of the chemical constituents of MDL.
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spelling pubmed-61553902018-11-13 Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS Wang, Jinfeng Jia, Ziyao Zhang, Zhihao Wang, Yutong Liu, Xi Wang, Linghua Lin, Ruichao Molecules Article The ethnic drug Melastoma dodecandrum Lour. (MDL) is widely distributed throughout South China, and is the major component of Gong Yan Ping Tablets/Capsules and Zi Di Ning Xue San. Although the pharmacological effects of MDL have been well documented, its chemical profile has not been fully determined. In this study, we have developed a rapid and sensitive UPLC-ESI-Q-Exactive Focus-MS/MS method to characterize the chemical constituents of MDL in the positive and negative ionization modes. A comparison of the chromatographic and spectrometric data obtained using this method with data from databases, the literature and reference standards allowed us to identify or tentatively characterize 109 compounds, including 26 fatty acids, 26 organic acids, 33 flavonoids, six tannins, 10 triterpenoids, two steroids and six other compounds. Notably, 55 of the compounds characterized in this study have never been detected before in this plant. The information obtained in this study therefore enriches our understanding of the chemical composition of MDL and could be used in quality control, pharmacological research and the development of drugs based on MDL. In addition, this study represents the first reported comprehensive analysis of the chemical constituents of MDL. MDPI 2017-03-17 /pmc/articles/PMC6155390/ /pubmed/28304342 http://dx.doi.org/10.3390/molecules22030476 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jinfeng
Jia, Ziyao
Zhang, Zhihao
Wang, Yutong
Liu, Xi
Wang, Linghua
Lin, Ruichao
Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS
title Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS
title_full Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS
title_fullStr Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS
title_full_unstemmed Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS
title_short Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS
title_sort analysis of chemical constituents of melastoma dodecandrum lour. by uplc-esi-q-exactive focus-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155390/
https://www.ncbi.nlm.nih.gov/pubmed/28304342
http://dx.doi.org/10.3390/molecules22030476
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