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Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells
Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155625/ https://www.ncbi.nlm.nih.gov/pubmed/28117734 http://dx.doi.org/10.3390/molecules22010167 |
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author | Imreova, Petronela Feruszova, Jana Kyzek, Stanislav Bodnarova, Kristina Zduriencikova, Martina Kozics, Katarina Mucaji, Pavel Galova, Eliska Sevcovicova, Andrea Miadokova, Eva Chalupa, Ivan |
author_facet | Imreova, Petronela Feruszova, Jana Kyzek, Stanislav Bodnarova, Kristina Zduriencikova, Martina Kozics, Katarina Mucaji, Pavel Galova, Eliska Sevcovicova, Andrea Miadokova, Eva Chalupa, Ivan |
author_sort | Imreova, Petronela |
collection | PubMed |
description | Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity of HF has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. The present work is designed to investigate the potential antigenotoxic effects of HF using three different experimental test systems. The antigenotoxic effect of HF leading to the decrease of primary/transient promutagenic genetic changes was detected by the alkaline comet assay on human lymphocytes. The HF antimutagenic effect leading to the reduction of gene mutations was assessed using the Ames test on the standard Salmonella typhimurium (TA97, TA98, and TA100) bacterial strains, and the anticlastogenic effect of HF leading to the reduction of chromosome aberrations was evaluated by the in vitro mammalian chromosome aberration test on the human tumor cell line HepG2 and the non-carcinogenic cell line VH10. Our findings provided evidence that HF showed antigenotoxic effects towards oxidative mutagen zeocin in the comet assay and diagnostic mutagen (4-nitroquinoline-1-oxide) in the Ames test. Moreover, HF exhibited an anticlastogenic effect towards benzo(a)pyrene and cisplatin in the chromosome aberration test. |
format | Online Article Text |
id | pubmed-6155625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61556252018-11-13 Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells Imreova, Petronela Feruszova, Jana Kyzek, Stanislav Bodnarova, Kristina Zduriencikova, Martina Kozics, Katarina Mucaji, Pavel Galova, Eliska Sevcovicova, Andrea Miadokova, Eva Chalupa, Ivan Molecules Article Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity of HF has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. The present work is designed to investigate the potential antigenotoxic effects of HF using three different experimental test systems. The antigenotoxic effect of HF leading to the decrease of primary/transient promutagenic genetic changes was detected by the alkaline comet assay on human lymphocytes. The HF antimutagenic effect leading to the reduction of gene mutations was assessed using the Ames test on the standard Salmonella typhimurium (TA97, TA98, and TA100) bacterial strains, and the anticlastogenic effect of HF leading to the reduction of chromosome aberrations was evaluated by the in vitro mammalian chromosome aberration test on the human tumor cell line HepG2 and the non-carcinogenic cell line VH10. Our findings provided evidence that HF showed antigenotoxic effects towards oxidative mutagen zeocin in the comet assay and diagnostic mutagen (4-nitroquinoline-1-oxide) in the Ames test. Moreover, HF exhibited an anticlastogenic effect towards benzo(a)pyrene and cisplatin in the chromosome aberration test. MDPI 2017-01-21 /pmc/articles/PMC6155625/ /pubmed/28117734 http://dx.doi.org/10.3390/molecules22010167 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Imreova, Petronela Feruszova, Jana Kyzek, Stanislav Bodnarova, Kristina Zduriencikova, Martina Kozics, Katarina Mucaji, Pavel Galova, Eliska Sevcovicova, Andrea Miadokova, Eva Chalupa, Ivan Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
title | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
title_full | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
title_fullStr | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
title_full_unstemmed | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
title_short | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
title_sort | hyperforin exhibits antigenotoxic activity on human and bacterial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155625/ https://www.ncbi.nlm.nih.gov/pubmed/28117734 http://dx.doi.org/10.3390/molecules22010167 |
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