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Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities
The aim of this research was to perform a phytochemical study of the methanol leaves extract of T. guianensis (MET) guided by vasodilatory and antioxidant activities. The chemical profile of MET and the ethyl acetate fraction (EA fraction) was determined by HPLC-UV-MS and EA fraction guided fraction...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155791/ https://www.ncbi.nlm.nih.gov/pubmed/28218702 http://dx.doi.org/10.3390/molecules22020304 |
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author | Rodrigues, Amélia M. G. Guimarães, Denise O. Konno, Tatiana U. P. Tinoco, Luzineide W. Barth, Thiago Aguiar, Fernando A. Lopes, Norberto P. Leal, Ivana C. R. Raimundo, Juliana M. Muzitano, Michelle F. |
author_facet | Rodrigues, Amélia M. G. Guimarães, Denise O. Konno, Tatiana U. P. Tinoco, Luzineide W. Barth, Thiago Aguiar, Fernando A. Lopes, Norberto P. Leal, Ivana C. R. Raimundo, Juliana M. Muzitano, Michelle F. |
author_sort | Rodrigues, Amélia M. G. |
collection | PubMed |
description | The aim of this research was to perform a phytochemical study of the methanol leaves extract of T. guianensis (MET) guided by vasodilatory and antioxidant activities. The chemical profile of MET and the ethyl acetate fraction (EA fraction) was determined by HPLC-UV-MS and EA fraction guided fractionation by reverse-phase chromatography. The vasorelaxant effects of MET, fractions, sub-fractions and constituents were assessed on rat aorta pre-contracted with phenylephrine. Antioxidant activity was evaluated by using a DPPH assay. The results show that MET-induced vasodilation was dependent on NO/cGMP; and that the PI3K/Akt pathway seems to be the main route involved in eNOS activation. The EA fraction showed greater vasodilatory and antioxidant potency and was submitted to further fractionation. This allowed the isolation and characterization of quercetin, quercetin 3-O-(6″-O-galloyl)-β-d-galactopyranoside and 1,4,6-tri-O-galloyl-β-d-glucose. Also, galloyl-HHDP-hexoside and myricetin deoxyhexoside were identified by HPLC-UV-MS. These compounds are being described for the first time for T. guianensis. 1,4,6-tri-O-galloyl-β-d-glucose and quercetin 3-O-(6″-O-galloyl)-β-d-galactopyranoside showed no vasodilatory activity. Quercetin and myricetin glycoside seems to contribute to the MET activity, since they have been reported as vasodilatory flavonoids. MET-induced vasodilation could contribute to the hypotensive effect of T. guianensis previously reported. |
format | Online Article Text |
id | pubmed-6155791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61557912018-11-13 Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities Rodrigues, Amélia M. G. Guimarães, Denise O. Konno, Tatiana U. P. Tinoco, Luzineide W. Barth, Thiago Aguiar, Fernando A. Lopes, Norberto P. Leal, Ivana C. R. Raimundo, Juliana M. Muzitano, Michelle F. Molecules Article The aim of this research was to perform a phytochemical study of the methanol leaves extract of T. guianensis (MET) guided by vasodilatory and antioxidant activities. The chemical profile of MET and the ethyl acetate fraction (EA fraction) was determined by HPLC-UV-MS and EA fraction guided fractionation by reverse-phase chromatography. The vasorelaxant effects of MET, fractions, sub-fractions and constituents were assessed on rat aorta pre-contracted with phenylephrine. Antioxidant activity was evaluated by using a DPPH assay. The results show that MET-induced vasodilation was dependent on NO/cGMP; and that the PI3K/Akt pathway seems to be the main route involved in eNOS activation. The EA fraction showed greater vasodilatory and antioxidant potency and was submitted to further fractionation. This allowed the isolation and characterization of quercetin, quercetin 3-O-(6″-O-galloyl)-β-d-galactopyranoside and 1,4,6-tri-O-galloyl-β-d-glucose. Also, galloyl-HHDP-hexoside and myricetin deoxyhexoside were identified by HPLC-UV-MS. These compounds are being described for the first time for T. guianensis. 1,4,6-tri-O-galloyl-β-d-glucose and quercetin 3-O-(6″-O-galloyl)-β-d-galactopyranoside showed no vasodilatory activity. Quercetin and myricetin glycoside seems to contribute to the MET activity, since they have been reported as vasodilatory flavonoids. MET-induced vasodilation could contribute to the hypotensive effect of T. guianensis previously reported. MDPI 2017-02-18 /pmc/articles/PMC6155791/ /pubmed/28218702 http://dx.doi.org/10.3390/molecules22020304 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rodrigues, Amélia M. G. Guimarães, Denise O. Konno, Tatiana U. P. Tinoco, Luzineide W. Barth, Thiago Aguiar, Fernando A. Lopes, Norberto P. Leal, Ivana C. R. Raimundo, Juliana M. Muzitano, Michelle F. Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities |
title | Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities |
title_full | Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities |
title_fullStr | Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities |
title_full_unstemmed | Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities |
title_short | Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities |
title_sort | phytochemical study of tapirira guianensis leaves guided by vasodilatory and antioxidant activities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155791/ https://www.ncbi.nlm.nih.gov/pubmed/28218702 http://dx.doi.org/10.3390/molecules22020304 |
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