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Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites

Lipases from Candida antarctica (isoform B) and Rhizomucor miehei (CALB and RML) have been immobilized on octyl-agarose (OC) and further coated with polyethylenimine (PEI) and dextran sulfate (DS). The enzymes just immobilized on OC supports could be easily released from the support using 2% SDS at...

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Detalles Bibliográficos
Autores principales: Virgen-Ortíz, Jose J., Pedrero, Sara G., Fernandez-Lopez, Laura, Lopez-Carrobles, Nerea, Gorines, Beatriz C., Otero, Cristina, Fernandez-Lafuente, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155924/
https://www.ncbi.nlm.nih.gov/pubmed/28067789
http://dx.doi.org/10.3390/molecules22010091