SK2 Channels Associate With mGlu(1α) Receptors and Ca(V)2.1 Channels in Purkinje Cells

The small-conductance, Ca(2+)-activated K(+) (SK) channel subtype SK2 regulates the spike rate and firing frequency, as well as Ca(2+) transients in Purkinje cells (PCs). To understand the molecular basis by which SK2 channels mediate these functions, we analyzed the exact location and densities of SK2 channels along the neuronal surface of the mouse cerebellar PCs using SDS-digested freeze-fracture replica labeling (SDS-FRL) of high sensitivity combined with quantitative analyses. Immunogold particles for SK2 were observed on post- and pre-synaptic compartments showing both scattered and clustered distribution patterns. We found an axo-somato-dendritic gradient of the SK2 particle density increasing 12-fold from soma to dendritic spines. Using two different immunogold approaches, we also found that SK2 immunoparticles were frequently adjacent to, but never overlap with, the postsynaptic density of excitatory synapses in PC spines. Co-immunoprecipitation analysis demonstrated that SK2 channels form macromolecular complexes with two types of proteins that mobilize Ca(2+): Ca(V)2.1 channels and mGlu(1α) receptors in the cerebellum. Freeze-fracture replica double-labeling showed significant co-clustering of particles for SK2 with those for Ca(V)2.1 channels and mGlu(1α) receptors. SK2 channels were also detected at presynaptic sites, mostly at the presynaptic active zone (AZ), where they are close to Ca(V)2.1 channels, though they are not significantly co-clustered. These data demonstrate that SK2 channels located in different neuronal compartments can associate with distinct proteins mobilizing Ca(2+), and suggest that the ultrastructural association of SK2 with Ca(V)2.1 and mGlu(1α) provides the mechanism that ensures voltage (excitability) regulation by distinct intracellular Ca(2+) transients in PCs.