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The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses

Pythium oligandrum (Oomycota) is known for its strong mycoparasitism against more than 50 fungal and oomycete species. However, the ability of this oomycete to suppress and kill the causal agents of dermatophytoses is yet to be studied. We provide a complex study of the interactions between P. oliga...

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Autores principales: Gabrielová, Alena, Mencl, Karel, Suchánek, Martin, Klimeš, Radim, Hubka, Vít, Kolařík, Miroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156753/
https://www.ncbi.nlm.nih.gov/pubmed/29967972
http://dx.doi.org/10.1007/s11046-018-0277-2
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author Gabrielová, Alena
Mencl, Karel
Suchánek, Martin
Klimeš, Radim
Hubka, Vít
Kolařík, Miroslav
author_facet Gabrielová, Alena
Mencl, Karel
Suchánek, Martin
Klimeš, Radim
Hubka, Vít
Kolařík, Miroslav
author_sort Gabrielová, Alena
collection PubMed
description Pythium oligandrum (Oomycota) is known for its strong mycoparasitism against more than 50 fungal and oomycete species. However, the ability of this oomycete to suppress and kill the causal agents of dermatophytoses is yet to be studied. We provide a complex study of the interactions between P. oligandrum and dermatophytes representing all species dominating in the developed countries. We assessed its biocidal potential by performing growth tests, on both solid and liquid cultivation media and by conducting a pilot clinical study. In addition, we studied the molecular background of mycoparasitism using expression profiles of genes responsible for the attack on the side of P. oligandrum and the stress response on the side of Microsporum canis. We showed that dermatophytes are efficiently suppressed or killed by P. oligandrum in the artificial conditions of cultivations media between 48 and 72 h after first contact. Significant intra- and interspecies variability was noted. Of the 69 patients included in the acute regimen study, symptoms were completely eliminated in 79% of the patients suffering from foot odour, hyperhidrosis disappeared in 67% of cases, clinical signs of dermatomycoses could no longer be observed in 83% of patients, and 15% of persons were relieved of symptoms of onychomycosis. Our investigations provide clear evidence that the oomycete is able to recognize and kill dermatophytes using recognition mechanisms that resemble those described in oomycetes attacking fungi infecting plants, albeit with some notable differences. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11046-018-0277-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-61567532018-10-10 The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses Gabrielová, Alena Mencl, Karel Suchánek, Martin Klimeš, Radim Hubka, Vít Kolařík, Miroslav Mycopathologia Original Article Pythium oligandrum (Oomycota) is known for its strong mycoparasitism against more than 50 fungal and oomycete species. However, the ability of this oomycete to suppress and kill the causal agents of dermatophytoses is yet to be studied. We provide a complex study of the interactions between P. oligandrum and dermatophytes representing all species dominating in the developed countries. We assessed its biocidal potential by performing growth tests, on both solid and liquid cultivation media and by conducting a pilot clinical study. In addition, we studied the molecular background of mycoparasitism using expression profiles of genes responsible for the attack on the side of P. oligandrum and the stress response on the side of Microsporum canis. We showed that dermatophytes are efficiently suppressed or killed by P. oligandrum in the artificial conditions of cultivations media between 48 and 72 h after first contact. Significant intra- and interspecies variability was noted. Of the 69 patients included in the acute regimen study, symptoms were completely eliminated in 79% of the patients suffering from foot odour, hyperhidrosis disappeared in 67% of cases, clinical signs of dermatomycoses could no longer be observed in 83% of patients, and 15% of persons were relieved of symptoms of onychomycosis. Our investigations provide clear evidence that the oomycete is able to recognize and kill dermatophytes using recognition mechanisms that resemble those described in oomycetes attacking fungi infecting plants, albeit with some notable differences. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11046-018-0277-2) contains supplementary material, which is available to authorized users. Springer Netherlands 2018-07-02 2018 /pmc/articles/PMC6156753/ /pubmed/29967972 http://dx.doi.org/10.1007/s11046-018-0277-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Gabrielová, Alena
Mencl, Karel
Suchánek, Martin
Klimeš, Radim
Hubka, Vít
Kolařík, Miroslav
The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
title The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
title_full The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
title_fullStr The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
title_full_unstemmed The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
title_short The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
title_sort oomycete pythium oligandrum can suppress and kill the causative agents of dermatophytoses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156753/
https://www.ncbi.nlm.nih.gov/pubmed/29967972
http://dx.doi.org/10.1007/s11046-018-0277-2
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