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Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides

PURPOSE: Personalized peptide-based cancer vaccines will be composed of multiple patient specific synthetic long peptides (SLPs) which may have various physicochemical properties. To formulate such SLPs, a flexible vaccine delivery system is required. We studied whether cationic liposomes are suitab...

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Autores principales: Heuts, Jeroen, Varypataki, Eleni Maria, van der Maaden, Koen, Romeijn, Stefan, Drijfhout, Jan Wouter, van Scheltinga, Anton Terwisscha, Ossendorp, Ferry, Jiskoot, Wim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156754/
https://www.ncbi.nlm.nih.gov/pubmed/30209623
http://dx.doi.org/10.1007/s11095-018-2490-6
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author Heuts, Jeroen
Varypataki, Eleni Maria
van der Maaden, Koen
Romeijn, Stefan
Drijfhout, Jan Wouter
van Scheltinga, Anton Terwisscha
Ossendorp, Ferry
Jiskoot, Wim
author_facet Heuts, Jeroen
Varypataki, Eleni Maria
van der Maaden, Koen
Romeijn, Stefan
Drijfhout, Jan Wouter
van Scheltinga, Anton Terwisscha
Ossendorp, Ferry
Jiskoot, Wim
author_sort Heuts, Jeroen
collection PubMed
description PURPOSE: Personalized peptide-based cancer vaccines will be composed of multiple patient specific synthetic long peptides (SLPs) which may have various physicochemical properties. To formulate such SLPs, a flexible vaccine delivery system is required. We studied whether cationic liposomes are suitable for this purpose. METHODS: Fifteen SIINFEKL T cell epitope-containing SLPs, widely differing in hydrophobicity and isoelectric point, were separately loaded in cationic liposomes via the dehydration-rehydration method. Particle size and polydispersity index (PDI) were measured via dynamic light scattering (DLS), and zeta potential with laser Doppler electrophoresis. Peptide loading was fluorescently determined and the immunogenicity of the formulated peptides was assessed in co-cultures of dendritic cells (DCs) and CD8(+) T-cells in vitro. RESULTS: All SLPs were loaded in cationic liposomes by using three different loading method variants, depending on the SLP characteristics. The fifteen liposomal formulations had a comparable size (< 200 nm), PDI (< 0.3) and zeta potential (22–30 mV). Cationic liposomes efficiently delivered the SLPs to DCs that subsequently activated SIINFEKL-specific CD8(+) T-cells, indicating improved immunological activity of the SLPs. CONCLUSION: Cationic liposomes can accommodate a wide range of different SLPs and are therefore a potential delivery platform for personalized cancer vaccines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11095-018-2490-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-61567542018-10-10 Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides Heuts, Jeroen Varypataki, Eleni Maria van der Maaden, Koen Romeijn, Stefan Drijfhout, Jan Wouter van Scheltinga, Anton Terwisscha Ossendorp, Ferry Jiskoot, Wim Pharm Res Research Paper PURPOSE: Personalized peptide-based cancer vaccines will be composed of multiple patient specific synthetic long peptides (SLPs) which may have various physicochemical properties. To formulate such SLPs, a flexible vaccine delivery system is required. We studied whether cationic liposomes are suitable for this purpose. METHODS: Fifteen SIINFEKL T cell epitope-containing SLPs, widely differing in hydrophobicity and isoelectric point, were separately loaded in cationic liposomes via the dehydration-rehydration method. Particle size and polydispersity index (PDI) were measured via dynamic light scattering (DLS), and zeta potential with laser Doppler electrophoresis. Peptide loading was fluorescently determined and the immunogenicity of the formulated peptides was assessed in co-cultures of dendritic cells (DCs) and CD8(+) T-cells in vitro. RESULTS: All SLPs were loaded in cationic liposomes by using three different loading method variants, depending on the SLP characteristics. The fifteen liposomal formulations had a comparable size (< 200 nm), PDI (< 0.3) and zeta potential (22–30 mV). Cationic liposomes efficiently delivered the SLPs to DCs that subsequently activated SIINFEKL-specific CD8(+) T-cells, indicating improved immunological activity of the SLPs. CONCLUSION: Cationic liposomes can accommodate a wide range of different SLPs and are therefore a potential delivery platform for personalized cancer vaccines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11095-018-2490-6) contains supplementary material, which is available to authorized users. Springer US 2018-09-12 2018 /pmc/articles/PMC6156754/ /pubmed/30209623 http://dx.doi.org/10.1007/s11095-018-2490-6 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Paper
Heuts, Jeroen
Varypataki, Eleni Maria
van der Maaden, Koen
Romeijn, Stefan
Drijfhout, Jan Wouter
van Scheltinga, Anton Terwisscha
Ossendorp, Ferry
Jiskoot, Wim
Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
title Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
title_full Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
title_fullStr Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
title_full_unstemmed Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
title_short Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides
title_sort cationic liposomes: a flexible vaccine delivery system for physicochemically diverse antigenic peptides
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156754/
https://www.ncbi.nlm.nih.gov/pubmed/30209623
http://dx.doi.org/10.1007/s11095-018-2490-6
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