Application of 2′-O-(2-N-Methylcarbamoylethyl) Nucleotides in RNase H-Dependent Antisense Oligonucleotides

An RNase H-dependent antisense oligonucleotide (ASO), having the 2′-O-(2-N-methylcarbamoylethyl) (MCE) modification, was evaluated in vitro and in vivo. The antisense activities of an ASO having the MCE modification were comparable with those of an ASO having the 2′-O-methoxyethyl (MOE) modification...

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Detalles Bibliográficos
Autores principales: Masaki, Yoshiaki, Iriyama, Yusuke, Nakajima, Hiroyuki, Kuroda, Yusuke, Kanaki, Tatsuro, Furukawa, Satoshi, Sekine, Mitsuo, Seio, Kohji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2018
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157346/
https://www.ncbi.nlm.nih.gov/pubmed/30020852
http://dx.doi.org/10.1089/nat.2018.0738
Descripción
Sumario:An RNase H-dependent antisense oligonucleotide (ASO), having the 2′-O-(2-N-methylcarbamoylethyl) (MCE) modification, was evaluated in vitro and in vivo. The antisense activities of an ASO having the MCE modification were comparable with those of an ASO having the 2′-O-methoxyethyl (MOE) modification in both in vitro and in vivo experiments. In contrast, the hepatotoxic potential of the ASO having the MCE modification was lower than that of the ASO having the MOE modification. Thus, these findings suggested that the MCE modification could be used as an alternative to the MOE modification.

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