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Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
The data in the present article are related to research article (doi: https://doi.org/10.1016/j.imlet.2018.04.002) [1]. The data describes the detailed immunization protocol for generating polyclonal antisera to murine erythrocytes in rat. The rat anti-mouse erythrocyte serum is then tested for its...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157386/ https://www.ncbi.nlm.nih.gov/pubmed/30263917 http://dx.doi.org/10.1016/j.dib.2018.08.192 |
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author | Sharma, Priyanka Puri, Niti |
author_facet | Sharma, Priyanka Puri, Niti |
author_sort | Sharma, Priyanka |
collection | PubMed |
description | The data in the present article are related to research article (doi: https://doi.org/10.1016/j.imlet.2018.04.002) [1]. The data describes the detailed immunization protocol for generating polyclonal antisera to murine erythrocytes in rat. The rat anti-mouse erythrocyte serum is then tested for its ability to bind and opsonize murine erythrocytes. Second set of data confirms the oxidative damage to murine erythrocytes by treatment with different dose of the tert-butyl hydroperoxide (t-BHP) on the basis of phosphotidylserine externalization by murine erythrocytes as well as measurement of reactive oxygen species (ROS) formation in t-BHP treated erythrocytes. Third set of data depicts lack of mast cell degranulation in the form of β- hexosaminidase release in response to co-incubation of mast cell with normal and oxidatively damaged erythrocytes. Lastly, the uptake of oxidatively damaged erythrocytes by resting and activated RBL-2H3 mast cells is shown by live cell imaging using confocal microscope. |
format | Online Article Text |
id | pubmed-6157386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61573862018-09-27 Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells Sharma, Priyanka Puri, Niti Data Brief Immunology and Microbiology The data in the present article are related to research article (doi: https://doi.org/10.1016/j.imlet.2018.04.002) [1]. The data describes the detailed immunization protocol for generating polyclonal antisera to murine erythrocytes in rat. The rat anti-mouse erythrocyte serum is then tested for its ability to bind and opsonize murine erythrocytes. Second set of data confirms the oxidative damage to murine erythrocytes by treatment with different dose of the tert-butyl hydroperoxide (t-BHP) on the basis of phosphotidylserine externalization by murine erythrocytes as well as measurement of reactive oxygen species (ROS) formation in t-BHP treated erythrocytes. Third set of data depicts lack of mast cell degranulation in the form of β- hexosaminidase release in response to co-incubation of mast cell with normal and oxidatively damaged erythrocytes. Lastly, the uptake of oxidatively damaged erythrocytes by resting and activated RBL-2H3 mast cells is shown by live cell imaging using confocal microscope. Elsevier 2018-09-13 /pmc/articles/PMC6157386/ /pubmed/30263917 http://dx.doi.org/10.1016/j.dib.2018.08.192 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Immunology and Microbiology Sharma, Priyanka Puri, Niti Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
title | Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
title_full | Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
title_fullStr | Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
title_full_unstemmed | Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
title_short | Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
title_sort | data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells |
topic | Immunology and Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157386/ https://www.ncbi.nlm.nih.gov/pubmed/30263917 http://dx.doi.org/10.1016/j.dib.2018.08.192 |
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