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Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells

The data in the present article are related to research article (doi: https://doi.org/10.1016/j.imlet.2018.04.002) [1]. The data describes the detailed immunization protocol for generating polyclonal antisera to murine erythrocytes in rat. The rat anti-mouse erythrocyte serum is then tested for its...

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Autores principales: Sharma, Priyanka, Puri, Niti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157386/
https://www.ncbi.nlm.nih.gov/pubmed/30263917
http://dx.doi.org/10.1016/j.dib.2018.08.192
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author Sharma, Priyanka
Puri, Niti
author_facet Sharma, Priyanka
Puri, Niti
author_sort Sharma, Priyanka
collection PubMed
description The data in the present article are related to research article (doi: https://doi.org/10.1016/j.imlet.2018.04.002) [1]. The data describes the detailed immunization protocol for generating polyclonal antisera to murine erythrocytes in rat. The rat anti-mouse erythrocyte serum is then tested for its ability to bind and opsonize murine erythrocytes. Second set of data confirms the oxidative damage to murine erythrocytes by treatment with different dose of the tert-butyl hydroperoxide (t-BHP) on the basis of phosphotidylserine externalization by murine erythrocytes as well as measurement of reactive oxygen species (ROS) formation in t-BHP treated erythrocytes. Third set of data depicts lack of mast cell degranulation in the form of β- hexosaminidase release in response to co-incubation of mast cell with normal and oxidatively damaged erythrocytes. Lastly, the uptake of oxidatively damaged erythrocytes by resting and activated RBL-2H3 mast cells is shown by live cell imaging using confocal microscope.
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spelling pubmed-61573862018-09-27 Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells Sharma, Priyanka Puri, Niti Data Brief Immunology and Microbiology The data in the present article are related to research article (doi: https://doi.org/10.1016/j.imlet.2018.04.002) [1]. The data describes the detailed immunization protocol for generating polyclonal antisera to murine erythrocytes in rat. The rat anti-mouse erythrocyte serum is then tested for its ability to bind and opsonize murine erythrocytes. Second set of data confirms the oxidative damage to murine erythrocytes by treatment with different dose of the tert-butyl hydroperoxide (t-BHP) on the basis of phosphotidylserine externalization by murine erythrocytes as well as measurement of reactive oxygen species (ROS) formation in t-BHP treated erythrocytes. Third set of data depicts lack of mast cell degranulation in the form of β- hexosaminidase release in response to co-incubation of mast cell with normal and oxidatively damaged erythrocytes. Lastly, the uptake of oxidatively damaged erythrocytes by resting and activated RBL-2H3 mast cells is shown by live cell imaging using confocal microscope. Elsevier 2018-09-13 /pmc/articles/PMC6157386/ /pubmed/30263917 http://dx.doi.org/10.1016/j.dib.2018.08.192 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Immunology and Microbiology
Sharma, Priyanka
Puri, Niti
Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
title Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
title_full Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
title_fullStr Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
title_full_unstemmed Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
title_short Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
title_sort data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
topic Immunology and Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157386/
https://www.ncbi.nlm.nih.gov/pubmed/30263917
http://dx.doi.org/10.1016/j.dib.2018.08.192
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