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Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes

Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in w...

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Detalles Bibliográficos
Autores principales: Jia, Zhiming, Guo, Hailin, Xie, Hua, Bao, Xingqi, Huang, Yichen, Yang, Ganggang, Chen, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157888/
https://www.ncbi.nlm.nih.gov/pubmed/30256839
http://dx.doi.org/10.1371/journal.pone.0204677
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author Jia, Zhiming
Guo, Hailin
Xie, Hua
Bao, Xingqi
Huang, Yichen
Yang, Ganggang
Chen, Fang
author_facet Jia, Zhiming
Guo, Hailin
Xie, Hua
Bao, Xingqi
Huang, Yichen
Yang, Ganggang
Chen, Fang
author_sort Jia, Zhiming
collection PubMed
description Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on common polystyrene dishes at a ratio of 6:1 for 5 to 6 days; the method is simple, cost-effective and highly repeatable. Moreover, the cocultured cell sheet contained capillary-like networks and could secrete a variety of angiogenic factors. Finally, in vivo studies proved that the cocultured cell sheets were more favorable for the fabrication of vascularized smooth muscle tissues compared to single SMC sheets. This study provides a promising avenue for smooth muscle tissue engineering.
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spelling pubmed-61578882018-10-19 Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes Jia, Zhiming Guo, Hailin Xie, Hua Bao, Xingqi Huang, Yichen Yang, Ganggang Chen, Fang PLoS One Research Article Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on common polystyrene dishes at a ratio of 6:1 for 5 to 6 days; the method is simple, cost-effective and highly repeatable. Moreover, the cocultured cell sheet contained capillary-like networks and could secrete a variety of angiogenic factors. Finally, in vivo studies proved that the cocultured cell sheets were more favorable for the fabrication of vascularized smooth muscle tissues compared to single SMC sheets. This study provides a promising avenue for smooth muscle tissue engineering. Public Library of Science 2018-09-26 /pmc/articles/PMC6157888/ /pubmed/30256839 http://dx.doi.org/10.1371/journal.pone.0204677 Text en © 2018 Jia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jia, Zhiming
Guo, Hailin
Xie, Hua
Bao, Xingqi
Huang, Yichen
Yang, Ganggang
Chen, Fang
Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
title Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
title_full Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
title_fullStr Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
title_full_unstemmed Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
title_short Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
title_sort harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157888/
https://www.ncbi.nlm.nih.gov/pubmed/30256839
http://dx.doi.org/10.1371/journal.pone.0204677
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