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Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes
Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in w...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157888/ https://www.ncbi.nlm.nih.gov/pubmed/30256839 http://dx.doi.org/10.1371/journal.pone.0204677 |
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author | Jia, Zhiming Guo, Hailin Xie, Hua Bao, Xingqi Huang, Yichen Yang, Ganggang Chen, Fang |
author_facet | Jia, Zhiming Guo, Hailin Xie, Hua Bao, Xingqi Huang, Yichen Yang, Ganggang Chen, Fang |
author_sort | Jia, Zhiming |
collection | PubMed |
description | Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on common polystyrene dishes at a ratio of 6:1 for 5 to 6 days; the method is simple, cost-effective and highly repeatable. Moreover, the cocultured cell sheet contained capillary-like networks and could secrete a variety of angiogenic factors. Finally, in vivo studies proved that the cocultured cell sheets were more favorable for the fabrication of vascularized smooth muscle tissues compared to single SMC sheets. This study provides a promising avenue for smooth muscle tissue engineering. |
format | Online Article Text |
id | pubmed-6157888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61578882018-10-19 Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes Jia, Zhiming Guo, Hailin Xie, Hua Bao, Xingqi Huang, Yichen Yang, Ganggang Chen, Fang PLoS One Research Article Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on common polystyrene dishes at a ratio of 6:1 for 5 to 6 days; the method is simple, cost-effective and highly repeatable. Moreover, the cocultured cell sheet contained capillary-like networks and could secrete a variety of angiogenic factors. Finally, in vivo studies proved that the cocultured cell sheets were more favorable for the fabrication of vascularized smooth muscle tissues compared to single SMC sheets. This study provides a promising avenue for smooth muscle tissue engineering. Public Library of Science 2018-09-26 /pmc/articles/PMC6157888/ /pubmed/30256839 http://dx.doi.org/10.1371/journal.pone.0204677 Text en © 2018 Jia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jia, Zhiming Guo, Hailin Xie, Hua Bao, Xingqi Huang, Yichen Yang, Ganggang Chen, Fang Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
title | Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
title_full | Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
title_fullStr | Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
title_full_unstemmed | Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
title_short | Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
title_sort | harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157888/ https://www.ncbi.nlm.nih.gov/pubmed/30256839 http://dx.doi.org/10.1371/journal.pone.0204677 |
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