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Fecal microbiota profile in a group of myasthenia gravis patients
The intestinal microbiota plays a key role in the maintenance of human health. Alterations in this microbiota have been described in several autoimmune diseases, including nervous system diseases. Nevertheless, the information regarding neuromuscular conditions is still limited. In this study, we ai...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158187/ https://www.ncbi.nlm.nih.gov/pubmed/30258104 http://dx.doi.org/10.1038/s41598-018-32700-y |
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author | Moris, German Arboleya, Silvia Mancabelli, Leonardo Milani, Christian Ventura, Marco de los Reyes-Gavilán, Clara G. Gueimonde, Miguel |
author_facet | Moris, German Arboleya, Silvia Mancabelli, Leonardo Milani, Christian Ventura, Marco de los Reyes-Gavilán, Clara G. Gueimonde, Miguel |
author_sort | Moris, German |
collection | PubMed |
description | The intestinal microbiota plays a key role in the maintenance of human health. Alterations in this microbiota have been described in several autoimmune diseases, including nervous system diseases. Nevertheless, the information regarding neuromuscular conditions is still limited. In this study, we aimed at characterizing the intestinal microbiota composition in myasthenia gravis patients (MG). To this end fecal samples were taken from ten patients, with antibodies against the acetylcholine receptor, and ten age and sex matched controls from the same population (Asturias region, Spain). Fecal samples were submitted to microbiota analyses by 16S rRNA gene profiling, bifidobacterial ITS-region profiling and qPCR. The fecal levels of short chain fatty acids were determined by gas chromatography. MG patients were found to harbor lower relative proportions of Verrucomicrobiaceae and Bifidobacteriaceae, among others, and increased of the phylum Bacteroidetes and the family Desulfovibrionaceae. The increase of these latter microbial groups was also confirmed at quantitative level by qPCR. In contrast, no statistically significant differences were found between MG patients and the control group in the bifidobacterial population at the species level or in short chain fatty acids profiles. Our data indicates an altered fecal microbiota pattern in MG patients and point out at specific microbiota targets for intervention in this population. |
format | Online Article Text |
id | pubmed-6158187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61581872018-09-28 Fecal microbiota profile in a group of myasthenia gravis patients Moris, German Arboleya, Silvia Mancabelli, Leonardo Milani, Christian Ventura, Marco de los Reyes-Gavilán, Clara G. Gueimonde, Miguel Sci Rep Article The intestinal microbiota plays a key role in the maintenance of human health. Alterations in this microbiota have been described in several autoimmune diseases, including nervous system diseases. Nevertheless, the information regarding neuromuscular conditions is still limited. In this study, we aimed at characterizing the intestinal microbiota composition in myasthenia gravis patients (MG). To this end fecal samples were taken from ten patients, with antibodies against the acetylcholine receptor, and ten age and sex matched controls from the same population (Asturias region, Spain). Fecal samples were submitted to microbiota analyses by 16S rRNA gene profiling, bifidobacterial ITS-region profiling and qPCR. The fecal levels of short chain fatty acids were determined by gas chromatography. MG patients were found to harbor lower relative proportions of Verrucomicrobiaceae and Bifidobacteriaceae, among others, and increased of the phylum Bacteroidetes and the family Desulfovibrionaceae. The increase of these latter microbial groups was also confirmed at quantitative level by qPCR. In contrast, no statistically significant differences were found between MG patients and the control group in the bifidobacterial population at the species level or in short chain fatty acids profiles. Our data indicates an altered fecal microbiota pattern in MG patients and point out at specific microbiota targets for intervention in this population. Nature Publishing Group UK 2018-09-26 /pmc/articles/PMC6158187/ /pubmed/30258104 http://dx.doi.org/10.1038/s41598-018-32700-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Moris, German Arboleya, Silvia Mancabelli, Leonardo Milani, Christian Ventura, Marco de los Reyes-Gavilán, Clara G. Gueimonde, Miguel Fecal microbiota profile in a group of myasthenia gravis patients |
title | Fecal microbiota profile in a group of myasthenia gravis patients |
title_full | Fecal microbiota profile in a group of myasthenia gravis patients |
title_fullStr | Fecal microbiota profile in a group of myasthenia gravis patients |
title_full_unstemmed | Fecal microbiota profile in a group of myasthenia gravis patients |
title_short | Fecal microbiota profile in a group of myasthenia gravis patients |
title_sort | fecal microbiota profile in a group of myasthenia gravis patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158187/ https://www.ncbi.nlm.nih.gov/pubmed/30258104 http://dx.doi.org/10.1038/s41598-018-32700-y |
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