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Transgene-free genome editing in marine algae by bacterial conjugation – comparison with biolistic CRISPR/Cas9 transformation

The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle bombardment. Recently delivery by conjugation was added to the tool-box. An im...

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Detalles Bibliográficos
Autores principales:	Sharma, A. K., Nymark, M., Sparstad, T., Bones, A. M., Winge, P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158232/ https://www.ncbi.nlm.nih.gov/pubmed/30258061 http://dx.doi.org/10.1038/s41598-018-32342-0

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