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Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat

The current application of genome editing to crop plants is limited to cultivars that are amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing which does not require callus culture and regeneration. Shoot apical meristems (SAMs) contain a subepidermal cell layer...

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Detalles Bibliográficos
Autores principales: Hamada, Haruyasu, Liu, Yuelin, Nagira, Yozo, Miki, Ryuji, Taoka, Naoaki, Imai, Ryozo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158239/
https://www.ncbi.nlm.nih.gov/pubmed/30258105
http://dx.doi.org/10.1038/s41598-018-32714-6