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Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat
The current application of genome editing to crop plants is limited to cultivars that are amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing which does not require callus culture and regeneration. Shoot apical meristems (SAMs) contain a subepidermal cell layer...
Autores principales: | Hamada, Haruyasu, Liu, Yuelin, Nagira, Yozo, Miki, Ryuji, Taoka, Naoaki, Imai, Ryozo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158239/ https://www.ncbi.nlm.nih.gov/pubmed/30258105 http://dx.doi.org/10.1038/s41598-018-32714-6 |
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