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Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques

Atherosclerotic plaques constitute the primary cause of heart attack and stroke. However, we still lack a clear identification of the plaques. Here, we evaluate the feasibility of scanning acoustic microscopy (SAM) and time-resolved fluorescence spectroscopy (TRFS) in atherosclerotic plaque characte...

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Autores principales: Bilen, Bukem, Gokbulut, Belkis, Kafa, Ulku, Heves, Emre, Inci, Mehmet Naci, Unlu, Mehmet Burcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158264/
https://www.ncbi.nlm.nih.gov/pubmed/30258115
http://dx.doi.org/10.1038/s41598-018-32788-2
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author Bilen, Bukem
Gokbulut, Belkis
Kafa, Ulku
Heves, Emre
Inci, Mehmet Naci
Unlu, Mehmet Burcin
author_facet Bilen, Bukem
Gokbulut, Belkis
Kafa, Ulku
Heves, Emre
Inci, Mehmet Naci
Unlu, Mehmet Burcin
author_sort Bilen, Bukem
collection PubMed
description Atherosclerotic plaques constitute the primary cause of heart attack and stroke. However, we still lack a clear identification of the plaques. Here, we evaluate the feasibility of scanning acoustic microscopy (SAM) and time-resolved fluorescence spectroscopy (TRFS) in atherosclerotic plaque characterization. We perform dual-modality microscopic imaging of the human carotid atherosclerotic plaques. We first show that the acoustic impedance values are statistically higher in calcified regions compared with the collagen-rich areas. We then use CdTe/CdS quantum dots for imaging the atherosclerotic plaques by TRFS and show that fluorescence lifetime values of the quantum dots in collagen-rich areas are notably different from the ones in calcified areas. In summary, both modalities are successful in differentiating the calcified regions from the collagen-rich areas within the plaques indicating that these techniques are confirmatory and may be combined to characterize atherosclerotic plaques in the future.
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spelling pubmed-61582642018-09-28 Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques Bilen, Bukem Gokbulut, Belkis Kafa, Ulku Heves, Emre Inci, Mehmet Naci Unlu, Mehmet Burcin Sci Rep Article Atherosclerotic plaques constitute the primary cause of heart attack and stroke. However, we still lack a clear identification of the plaques. Here, we evaluate the feasibility of scanning acoustic microscopy (SAM) and time-resolved fluorescence spectroscopy (TRFS) in atherosclerotic plaque characterization. We perform dual-modality microscopic imaging of the human carotid atherosclerotic plaques. We first show that the acoustic impedance values are statistically higher in calcified regions compared with the collagen-rich areas. We then use CdTe/CdS quantum dots for imaging the atherosclerotic plaques by TRFS and show that fluorescence lifetime values of the quantum dots in collagen-rich areas are notably different from the ones in calcified areas. In summary, both modalities are successful in differentiating the calcified regions from the collagen-rich areas within the plaques indicating that these techniques are confirmatory and may be combined to characterize atherosclerotic plaques in the future. Nature Publishing Group UK 2018-09-26 /pmc/articles/PMC6158264/ /pubmed/30258115 http://dx.doi.org/10.1038/s41598-018-32788-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bilen, Bukem
Gokbulut, Belkis
Kafa, Ulku
Heves, Emre
Inci, Mehmet Naci
Unlu, Mehmet Burcin
Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques
title Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques
title_full Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques
title_fullStr Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques
title_full_unstemmed Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques
title_short Scanning Acoustic Microscopy and Time-Resolved Fluorescence Spectroscopy for Characterization of Atherosclerotic Plaques
title_sort scanning acoustic microscopy and time-resolved fluorescence spectroscopy for characterization of atherosclerotic plaques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158264/
https://www.ncbi.nlm.nih.gov/pubmed/30258115
http://dx.doi.org/10.1038/s41598-018-32788-2
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