Cargando…
Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling
Neuroinflammation plays an important role in secondary injury after traumatic brain injury (TBI). Andrographolide (Andro), a diterpenoid lactone isolated from Andrographis paniculata, has been demonstrated to exhibit anti-inflammatory activity in neurodegenerative disorders. This study therefore aim...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158349/ https://www.ncbi.nlm.nih.gov/pubmed/30294256 http://dx.doi.org/10.3389/fnins.2018.00657 |
_version_ | 1783358412732497920 |
---|---|
author | Tao, Li Zhang, Li Gao, Rong Jiang, Feng Cao, Jianbo Liu, Huixiang |
author_facet | Tao, Li Zhang, Li Gao, Rong Jiang, Feng Cao, Jianbo Liu, Huixiang |
author_sort | Tao, Li |
collection | PubMed |
description | Neuroinflammation plays an important role in secondary injury after traumatic brain injury (TBI). Andrographolide (Andro), a diterpenoid lactone isolated from Andrographis paniculata, has been demonstrated to exhibit anti-inflammatory activity in neurodegenerative disorders. This study therefore aimed to investigate the potential neuroprotective effects of Andro after TBI and explore the underlying mechanisms. In our study, we used a weight-dropped model to induce TBI in Sprague–Dawley rats, the neurological deficits were assessed using modified neurological severity scores, Fluoro-Jade B (FJB) and terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) staining were employed to examine neuronal degeneration and apoptosis after TBI, immunofluorescence was designed to investigate microglial activation. Quantitative Real-time PCR and ELISA were conducted to detect the expression levels of pro-inflammatory cytokines, Western blot was used to examine the expression level of proteins of relative signaling pathway. Our results showed that after Andro administration, the neurological deficit was attenuated, and the cerebral edema and apoptosis in brain tissues were also decreased following TBI. Both microglial activation and the expression of pro-inflammatory cytokines were significantly inhibited by Andro after TBI. Moreover, Andro inhibited NF-κB p65 subunit translocation and decreased the expression levels of phosphorylated extracellular signal regulated kinase (ERK) and p38 MAPK after TBI. Altogether, this study suggests that Andro could improve neurobehavioral function by inhibiting NF-κB and MAPK signaling pathway in TBI, which might provide a new approach for treating brain injury. |
format | Online Article Text |
id | pubmed-6158349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61583492018-10-05 Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling Tao, Li Zhang, Li Gao, Rong Jiang, Feng Cao, Jianbo Liu, Huixiang Front Neurosci Neuroscience Neuroinflammation plays an important role in secondary injury after traumatic brain injury (TBI). Andrographolide (Andro), a diterpenoid lactone isolated from Andrographis paniculata, has been demonstrated to exhibit anti-inflammatory activity in neurodegenerative disorders. This study therefore aimed to investigate the potential neuroprotective effects of Andro after TBI and explore the underlying mechanisms. In our study, we used a weight-dropped model to induce TBI in Sprague–Dawley rats, the neurological deficits were assessed using modified neurological severity scores, Fluoro-Jade B (FJB) and terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) staining were employed to examine neuronal degeneration and apoptosis after TBI, immunofluorescence was designed to investigate microglial activation. Quantitative Real-time PCR and ELISA were conducted to detect the expression levels of pro-inflammatory cytokines, Western blot was used to examine the expression level of proteins of relative signaling pathway. Our results showed that after Andro administration, the neurological deficit was attenuated, and the cerebral edema and apoptosis in brain tissues were also decreased following TBI. Both microglial activation and the expression of pro-inflammatory cytokines were significantly inhibited by Andro after TBI. Moreover, Andro inhibited NF-κB p65 subunit translocation and decreased the expression levels of phosphorylated extracellular signal regulated kinase (ERK) and p38 MAPK after TBI. Altogether, this study suggests that Andro could improve neurobehavioral function by inhibiting NF-κB and MAPK signaling pathway in TBI, which might provide a new approach for treating brain injury. Frontiers Media S.A. 2018-09-20 /pmc/articles/PMC6158349/ /pubmed/30294256 http://dx.doi.org/10.3389/fnins.2018.00657 Text en Copyright © 2018 Tao, Zhang, Gao, Jiang, Cao and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Tao, Li Zhang, Li Gao, Rong Jiang, Feng Cao, Jianbo Liu, Huixiang Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling |
title | Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling |
title_full | Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling |
title_fullStr | Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling |
title_full_unstemmed | Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling |
title_short | Andrographolide Alleviates Acute Brain Injury in a Rat Model of Traumatic Brain Injury: Possible Involvement of Inflammatory Signaling |
title_sort | andrographolide alleviates acute brain injury in a rat model of traumatic brain injury: possible involvement of inflammatory signaling |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158349/ https://www.ncbi.nlm.nih.gov/pubmed/30294256 http://dx.doi.org/10.3389/fnins.2018.00657 |
work_keys_str_mv | AT taoli andrographolidealleviatesacutebraininjuryinaratmodeloftraumaticbraininjurypossibleinvolvementofinflammatorysignaling AT zhangli andrographolidealleviatesacutebraininjuryinaratmodeloftraumaticbraininjurypossibleinvolvementofinflammatorysignaling AT gaorong andrographolidealleviatesacutebraininjuryinaratmodeloftraumaticbraininjurypossibleinvolvementofinflammatorysignaling AT jiangfeng andrographolidealleviatesacutebraininjuryinaratmodeloftraumaticbraininjurypossibleinvolvementofinflammatorysignaling AT caojianbo andrographolidealleviatesacutebraininjuryinaratmodeloftraumaticbraininjurypossibleinvolvementofinflammatorysignaling AT liuhuixiang andrographolidealleviatesacutebraininjuryinaratmodeloftraumaticbraininjurypossibleinvolvementofinflammatorysignaling |