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Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy
Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a method of choice to observe brain function with high frame rates at cellular resolution. Inherently to LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest a...
Autores principales: | Müllenbroich, M. Caroline, Turrini, Lapo, Silvestri, Ludovico, Alterini, Tommaso, Gheisari, Ali, Tiso, Natascia, Vanzi, Francesco, Sacconi, Leonardo, Pavone, Francesco S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158350/ https://www.ncbi.nlm.nih.gov/pubmed/30294262 http://dx.doi.org/10.3389/fncel.2018.00315 |
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