Cargando…

Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed

Rapeseed (Brassica napus L.) is a vital oil crop worldwide. High oleic acid content is a desirable quality trait for rapeseed oil, which makes it more beneficial to human health. However, many germplasm resources with high oleic acid content in rapeseed have not been evaluated with regard to their g...

Descripción completa

Detalles Bibliográficos
Autores principales: Long, Weihua, Hu, Maolong, Gao, Jianqin, Chen, Song, Zhang, Jiefu, Cheng, Li, Pu, Huiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158388/
https://www.ncbi.nlm.nih.gov/pubmed/30294343
http://dx.doi.org/10.3389/fgene.2018.00399
_version_ 1783358418432557056
author Long, Weihua
Hu, Maolong
Gao, Jianqin
Chen, Song
Zhang, Jiefu
Cheng, Li
Pu, Huiming
author_facet Long, Weihua
Hu, Maolong
Gao, Jianqin
Chen, Song
Zhang, Jiefu
Cheng, Li
Pu, Huiming
author_sort Long, Weihua
collection PubMed
description Rapeseed (Brassica napus L.) is a vital oil crop worldwide. High oleic acid content is a desirable quality trait for rapeseed oil, which makes it more beneficial to human health. However, many germplasm resources with high oleic acid content in rapeseed have not been evaluated with regard to their genotypes, making it difficult to select the best strains with this trait for the breeding of high oleic acid rapeseed variety. This work was to explore the gene-regulation mechanism of this trait using a new super-high oleic acid content (∼85%) line N1379T as genetic material. In this study, the sequences of four homologous fatty acid desaturase (BnFAD2) genes were compared between super-high (∼85%, N1379T) and normal (∼63%) oleic acid content lines. Results showed that there were two single-nucleotide polymorphisms (SNPs) in BnFAD2-1 and BnFAD2-2, respectively, which led to the amino acid changes (E106K and G303E) in the corresponding proteins. Functional analysis of both genes in yeast confirmed that these SNPs were loss-of-function mutations, thus limiting the conversion of oleic acid to linoleic acid and resulting in the considerable accumulation of oleic acid. Moreover, two specific cleaved amplified polymorphic sequences (CAPS) markers for the two SNPs were developed to identify genotypes of each line in the F(2) and BC(1) populations. Furthermore, these two mutant loci of BnFAD2-1 and BnFAD2-2 genes were positively associated with elevated oleic acid levels and had a similar effect with regard to the increase of oleic acid content. Taken together, these two novel SNPs in two different BnFAD2 genes jointly regulated the high oleic acid trait in this special germplasm. The study provided insight into the genetic regulation involved in oleic acid accumulation and highlighted the use of new alleles of BnFAD2-1 and BnFAD2-2 in breeding high oleic acid rapeseed varieties.
format Online
Article
Text
id pubmed-6158388
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-61583882018-10-05 Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed Long, Weihua Hu, Maolong Gao, Jianqin Chen, Song Zhang, Jiefu Cheng, Li Pu, Huiming Front Genet Genetics Rapeseed (Brassica napus L.) is a vital oil crop worldwide. High oleic acid content is a desirable quality trait for rapeseed oil, which makes it more beneficial to human health. However, many germplasm resources with high oleic acid content in rapeseed have not been evaluated with regard to their genotypes, making it difficult to select the best strains with this trait for the breeding of high oleic acid rapeseed variety. This work was to explore the gene-regulation mechanism of this trait using a new super-high oleic acid content (∼85%) line N1379T as genetic material. In this study, the sequences of four homologous fatty acid desaturase (BnFAD2) genes were compared between super-high (∼85%, N1379T) and normal (∼63%) oleic acid content lines. Results showed that there were two single-nucleotide polymorphisms (SNPs) in BnFAD2-1 and BnFAD2-2, respectively, which led to the amino acid changes (E106K and G303E) in the corresponding proteins. Functional analysis of both genes in yeast confirmed that these SNPs were loss-of-function mutations, thus limiting the conversion of oleic acid to linoleic acid and resulting in the considerable accumulation of oleic acid. Moreover, two specific cleaved amplified polymorphic sequences (CAPS) markers for the two SNPs were developed to identify genotypes of each line in the F(2) and BC(1) populations. Furthermore, these two mutant loci of BnFAD2-1 and BnFAD2-2 genes were positively associated with elevated oleic acid levels and had a similar effect with regard to the increase of oleic acid content. Taken together, these two novel SNPs in two different BnFAD2 genes jointly regulated the high oleic acid trait in this special germplasm. The study provided insight into the genetic regulation involved in oleic acid accumulation and highlighted the use of new alleles of BnFAD2-1 and BnFAD2-2 in breeding high oleic acid rapeseed varieties. Frontiers Media S.A. 2018-09-20 /pmc/articles/PMC6158388/ /pubmed/30294343 http://dx.doi.org/10.3389/fgene.2018.00399 Text en Copyright © 2018 Long, Hu, Gao, Chen, Zhang, Cheng and Pu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Long, Weihua
Hu, Maolong
Gao, Jianqin
Chen, Song
Zhang, Jiefu
Cheng, Li
Pu, Huiming
Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
title Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
title_full Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
title_fullStr Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
title_full_unstemmed Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
title_short Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
title_sort identification and functional analysis of two new mutant bnfad2 alleles that confer elevated oleic acid content in rapeseed
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158388/
https://www.ncbi.nlm.nih.gov/pubmed/30294343
http://dx.doi.org/10.3389/fgene.2018.00399
work_keys_str_mv AT longweihua identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed
AT humaolong identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed
AT gaojianqin identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed
AT chensong identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed
AT zhangjiefu identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed
AT chengli identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed
AT puhuiming identificationandfunctionalanalysisoftwonewmutantbnfad2allelesthatconferelevatedoleicacidcontentinrapeseed