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CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci

Nuclear organization has an important role in determining genome function; however, it is not clear how spatiotemporal organization of the genome relates to functionality. To elucidate this relationship, a method for tracking any locus of interest is desirable. Recently clustered regularly interspac...

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Detalles Bibliográficos
Autores principales: Tasan, Ipek, Sustackova, Gabriela, Zhang, Liguo, Kim, Jiah, Sivaguru, Mayandi, HamediRad, Mohammad, Wang, Yuchuan, Genova, Justin, Ma, Jian, Belmont, Andrew S, Zhao, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158506/
https://www.ncbi.nlm.nih.gov/pubmed/29912475
http://dx.doi.org/10.1093/nar/gky501