dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase

To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore. These dUTP derivatives and commercial Cy3− and...

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Detalles Bibliográficos
Autores principales: Zasedateleva, Olga A, Vasiliskov, Vadim A, Surzhikov, Sergey A, Kuznetsova, Viktoriya E, Shershov, Valeriy E, Guseinov, Timur O, Smirnov, Igor P, Yurasov, Roman A, Spitsyn, Maksim A, Chudinov, Alexander V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158613/
https://www.ncbi.nlm.nih.gov/pubmed/29648660
http://dx.doi.org/10.1093/nar/gky247

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