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Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro

Sense codon reassignment to unnatural amino acids (uAAs) represents a powerful approach for introducing novel properties into polypeptides. The main obstacle to this approach is competition between the native isoacceptor tRNA(s) and orthogonal tRNA(s) for the reassigned codon. While several chromato...

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Detalles Bibliográficos
Autores principales:	Cui, Zhenling, Wu, Yue, Mureev, Sergey, Alexandrov, Kirill
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158751/ https://www.ncbi.nlm.nih.gov/pubmed/29846683 http://dx.doi.org/10.1093/nar/gky365

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