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Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis)
BACKGROUND: VQ motif-containing (VQ) proteins are plant-specific proteins that interact with WRKY transcription factors and play important roles in plant growth, development and stress response. To date, VQ gene families have been identified and characterized in many plant species, including Arabido...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158892/ https://www.ncbi.nlm.nih.gov/pubmed/30257643 http://dx.doi.org/10.1186/s12864-018-5107-x |
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author | Guo, Junhong Chen, Jiangfei Yang, Jiankun Yu, Youben Yang, Yajun Wang, Weidong |
author_facet | Guo, Junhong Chen, Jiangfei Yang, Jiankun Yu, Youben Yang, Yajun Wang, Weidong |
author_sort | Guo, Junhong |
collection | PubMed |
description | BACKGROUND: VQ motif-containing (VQ) proteins are plant-specific proteins that interact with WRKY transcription factors and play important roles in plant growth, development and stress response. To date, VQ gene families have been identified and characterized in many plant species, including Arabidopsis, rice and grapevine. However, the VQ gene family in tea plant has not been reported, and the biological functions of this family remain unknown. RESULTS: In total, 25 CsVQ genes were identified based on the genome and transcriptome of tea plant, and a comprehensive bioinformatics analysis was performed. The CsVQ proteins all contained the typical conserved motif FxxhVQxhTG, and most proteins were localized in the nucleus. The phylogenetic analysis showed that the VQ proteins were classified into 5 groups (I, III-VI); the evolution of the CsVQ proteins is consistent with the evolutionary process of plants, and close proteins shared similar structures and functions. In addition, the expression analysis revealed that the CsVQ genes play important roles in the process of tea plant growth, development and response to salt and drought stress. Furthermore, a potential regulatory network including the interactions of CsVQ proteins with CsWRKY transcription factors and the regulation of upstream microRNA that is closely related to the above-mentioned processes is proposed. CONCLUSIONS: The results of this study increase our understanding and characterization of CsVQ genes and their encoded proteins in tea plant. This systematic analysis provided comprehensive information for further studies investigating the biological functions of CsVQ proteins in various developmental processes of tea plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5107-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6158892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61588922018-10-01 Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) Guo, Junhong Chen, Jiangfei Yang, Jiankun Yu, Youben Yang, Yajun Wang, Weidong BMC Genomics Research Article BACKGROUND: VQ motif-containing (VQ) proteins are plant-specific proteins that interact with WRKY transcription factors and play important roles in plant growth, development and stress response. To date, VQ gene families have been identified and characterized in many plant species, including Arabidopsis, rice and grapevine. However, the VQ gene family in tea plant has not been reported, and the biological functions of this family remain unknown. RESULTS: In total, 25 CsVQ genes were identified based on the genome and transcriptome of tea plant, and a comprehensive bioinformatics analysis was performed. The CsVQ proteins all contained the typical conserved motif FxxhVQxhTG, and most proteins were localized in the nucleus. The phylogenetic analysis showed that the VQ proteins were classified into 5 groups (I, III-VI); the evolution of the CsVQ proteins is consistent with the evolutionary process of plants, and close proteins shared similar structures and functions. In addition, the expression analysis revealed that the CsVQ genes play important roles in the process of tea plant growth, development and response to salt and drought stress. Furthermore, a potential regulatory network including the interactions of CsVQ proteins with CsWRKY transcription factors and the regulation of upstream microRNA that is closely related to the above-mentioned processes is proposed. CONCLUSIONS: The results of this study increase our understanding and characterization of CsVQ genes and their encoded proteins in tea plant. This systematic analysis provided comprehensive information for further studies investigating the biological functions of CsVQ proteins in various developmental processes of tea plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5107-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-26 /pmc/articles/PMC6158892/ /pubmed/30257643 http://dx.doi.org/10.1186/s12864-018-5107-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Guo, Junhong Chen, Jiangfei Yang, Jiankun Yu, Youben Yang, Yajun Wang, Weidong Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) |
title | Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) |
title_full | Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) |
title_fullStr | Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) |
title_full_unstemmed | Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) |
title_short | Identification, characterization and expression analysis of the VQ motif-containing gene family in tea plant (Camellia sinensis) |
title_sort | identification, characterization and expression analysis of the vq motif-containing gene family in tea plant (camellia sinensis) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158892/ https://www.ncbi.nlm.nih.gov/pubmed/30257643 http://dx.doi.org/10.1186/s12864-018-5107-x |
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