Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease

BACKGROUND: The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. RESULTS: The results revealed that...

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Autores principales: Qian, Kun, Tian, Xue, Shao, Hongxia, Ye, Jianqiang, Yao, Yongxiu, Nair, Venugopal, Qin, Aijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158924/
https://www.ncbi.nlm.nih.gov/pubmed/30257680
http://dx.doi.org/10.1186/s12917-018-1622-x
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author Qian, Kun
Tian, Xue
Shao, Hongxia
Ye, Jianqiang
Yao, Yongxiu
Nair, Venugopal
Qin, Aijian
author_facet Qian, Kun
Tian, Xue
Shao, Hongxia
Ye, Jianqiang
Yao, Yongxiu
Nair, Venugopal
Qin, Aijian
author_sort Qian, Kun
collection PubMed
description BACKGROUND: The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. RESULTS: The results revealed that monoclonal antibody (mAb) JE9 recognized (83)WDPQEL(88) motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J. CONCLUSIONS: A novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J.
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spelling pubmed-61589242018-10-01 Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease Qian, Kun Tian, Xue Shao, Hongxia Ye, Jianqiang Yao, Yongxiu Nair, Venugopal Qin, Aijian BMC Vet Res Research Article BACKGROUND: The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection. RESULTS: The results revealed that monoclonal antibody (mAb) JE9 recognized (83)WDPQEL(88) motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J. CONCLUSIONS: A novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J. BioMed Central 2018-09-26 /pmc/articles/PMC6158924/ /pubmed/30257680 http://dx.doi.org/10.1186/s12917-018-1622-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Qian, Kun
Tian, Xue
Shao, Hongxia
Ye, Jianqiang
Yao, Yongxiu
Nair, Venugopal
Qin, Aijian
Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
title Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
title_full Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
title_fullStr Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
title_full_unstemmed Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
title_short Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease
title_sort identification of novel b-cell epitope in gp85 of subgroup j avian leukosis virus and its application in diagnosis of disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158924/
https://www.ncbi.nlm.nih.gov/pubmed/30257680
http://dx.doi.org/10.1186/s12917-018-1622-x
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