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Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant

PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHO...

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Detalles Bibliográficos
Autores principales: Priyadi, Arief, Feng, Chao, Kang, Ming, Huang, Hongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159642/
https://www.ncbi.nlm.nih.gov/pubmed/30276026
http://dx.doi.org/10.1002/aps3.1178
Descripción
Sumario:PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHODS AND RESULTS: We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. CONCLUSIONS: These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta.