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Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant

PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHO...

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Autores principales: Priyadi, Arief, Feng, Chao, Kang, Ming, Huang, Hongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159642/
https://www.ncbi.nlm.nih.gov/pubmed/30276026
http://dx.doi.org/10.1002/aps3.1178
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author Priyadi, Arief
Feng, Chao
Kang, Ming
Huang, Hongwen
author_facet Priyadi, Arief
Feng, Chao
Kang, Ming
Huang, Hongwen
author_sort Priyadi, Arief
collection PubMed
description PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHODS AND RESULTS: We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. CONCLUSIONS: These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta.
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spelling pubmed-61596422018-10-01 Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant Priyadi, Arief Feng, Chao Kang, Ming Huang, Hongwen Appl Plant Sci Primer Notes PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHODS AND RESULTS: We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. CONCLUSIONS: These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta. John Wiley and Sons Inc. 2018-09-21 /pmc/articles/PMC6159642/ /pubmed/30276026 http://dx.doi.org/10.1002/aps3.1178 Text en © 2018 Priyadi et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primer Notes
Priyadi, Arief
Feng, Chao
Kang, Ming
Huang, Hongwen
Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
title Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
title_full Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
title_fullStr Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
title_full_unstemmed Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
title_short Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
title_sort development of 10 single‐copy nuclear dna markers for euchresta horsfieldii (fabaceae), a rare medicinal plant
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159642/
https://www.ncbi.nlm.nih.gov/pubmed/30276026
http://dx.doi.org/10.1002/aps3.1178
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