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Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHO...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159642/ https://www.ncbi.nlm.nih.gov/pubmed/30276026 http://dx.doi.org/10.1002/aps3.1178 |
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author | Priyadi, Arief Feng, Chao Kang, Ming Huang, Hongwen |
author_facet | Priyadi, Arief Feng, Chao Kang, Ming Huang, Hongwen |
author_sort | Priyadi, Arief |
collection | PubMed |
description | PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHODS AND RESULTS: We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. CONCLUSIONS: These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta. |
format | Online Article Text |
id | pubmed-6159642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61596422018-10-01 Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant Priyadi, Arief Feng, Chao Kang, Ming Huang, Hongwen Appl Plant Sci Primer Notes PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHODS AND RESULTS: We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. CONCLUSIONS: These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta. John Wiley and Sons Inc. 2018-09-21 /pmc/articles/PMC6159642/ /pubmed/30276026 http://dx.doi.org/10.1002/aps3.1178 Text en © 2018 Priyadi et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Primer Notes Priyadi, Arief Feng, Chao Kang, Ming Huang, Hongwen Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
title | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
title_full | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
title_fullStr | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
title_full_unstemmed | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
title_short | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
title_sort | development of 10 single‐copy nuclear dna markers for euchresta horsfieldii (fabaceae), a rare medicinal plant |
topic | Primer Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159642/ https://www.ncbi.nlm.nih.gov/pubmed/30276026 http://dx.doi.org/10.1002/aps3.1178 |
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