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Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection
BACKGROUND: V-set and immunoglobulin domain containing protein 4 (VSIG4) was reported to play an important role in tumorigenesis. However, the expression and clinical relevance in hepatocellular carcinoma (HCC) remain unknown. MATERIALS AND METHODS: First, the mRNA profiles of HCC were screened from...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159793/ https://www.ncbi.nlm.nih.gov/pubmed/30288101 http://dx.doi.org/10.2147/CMAR.S165822 |
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author | Zhu, Sicong Tan, Wenliang Li, Wenxin Zhou, Rui Wu, Xiaolin Chen, Xianqing Li, Wenda Shang, Changzhen Chen, Yajin |
author_facet | Zhu, Sicong Tan, Wenliang Li, Wenxin Zhou, Rui Wu, Xiaolin Chen, Xianqing Li, Wenda Shang, Changzhen Chen, Yajin |
author_sort | Zhu, Sicong |
collection | PubMed |
description | BACKGROUND: V-set and immunoglobulin domain containing protein 4 (VSIG4) was reported to play an important role in tumorigenesis. However, the expression and clinical relevance in hepatocellular carcinoma (HCC) remain unknown. MATERIALS AND METHODS: First, the mRNA profiles of HCC were screened from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus databases. VSIG4, a differentially expressed gene that has not been reported in HCC, was distinguished. Second, the correlation between VSIG4 expression and the prognosis of HCC patients from TCGA was analyzed. Third, VSIG4 mRNA level was detected in 36 pairs of HCC tissues and 4 HCC cell lines by PCR assay. And finally, prognosis analysis was assessed for 36 HCC patients with different expression levels of VSIG4. RESULTS: Bioinformatics analysis showed that VSIG4 expression was downregulated in HCC tissues, and the expression level of VSIG4 was negatively correlated with serum alpha fetal protein (AFP) level and tumor distant metastasis. Survival analysis of all HCC patients in TCGA indicated that the overall survival and disease-free survival were not significantly associated with VSIG4 expression. However, subgroup analysis showed that in the patients with hepatitis B virus-related HCC, both overall survival and disease-free survival were shorter in the low VSIG4 expression group. Our PCR results further showed that VSIG4 expression was significantly decreased in HCC tissues and HCC cell lines, and the disease-free survival in hepatitis B virus-related HCC patients with low VSIG4 expression was shorter than in those with high VSIG4 expression, which was consistent with the bioinformatics analysis results. CONCLUSION: Our study suggests that VSIG4 is downregulated in HCC, and low expression of VSIG4 is associated with poor prognosis in hepatitis B virus-related HCC patients. |
format | Online Article Text |
id | pubmed-6159793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61597932018-10-04 Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection Zhu, Sicong Tan, Wenliang Li, Wenxin Zhou, Rui Wu, Xiaolin Chen, Xianqing Li, Wenda Shang, Changzhen Chen, Yajin Cancer Manag Res Original Research BACKGROUND: V-set and immunoglobulin domain containing protein 4 (VSIG4) was reported to play an important role in tumorigenesis. However, the expression and clinical relevance in hepatocellular carcinoma (HCC) remain unknown. MATERIALS AND METHODS: First, the mRNA profiles of HCC were screened from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus databases. VSIG4, a differentially expressed gene that has not been reported in HCC, was distinguished. Second, the correlation between VSIG4 expression and the prognosis of HCC patients from TCGA was analyzed. Third, VSIG4 mRNA level was detected in 36 pairs of HCC tissues and 4 HCC cell lines by PCR assay. And finally, prognosis analysis was assessed for 36 HCC patients with different expression levels of VSIG4. RESULTS: Bioinformatics analysis showed that VSIG4 expression was downregulated in HCC tissues, and the expression level of VSIG4 was negatively correlated with serum alpha fetal protein (AFP) level and tumor distant metastasis. Survival analysis of all HCC patients in TCGA indicated that the overall survival and disease-free survival were not significantly associated with VSIG4 expression. However, subgroup analysis showed that in the patients with hepatitis B virus-related HCC, both overall survival and disease-free survival were shorter in the low VSIG4 expression group. Our PCR results further showed that VSIG4 expression was significantly decreased in HCC tissues and HCC cell lines, and the disease-free survival in hepatitis B virus-related HCC patients with low VSIG4 expression was shorter than in those with high VSIG4 expression, which was consistent with the bioinformatics analysis results. CONCLUSION: Our study suggests that VSIG4 is downregulated in HCC, and low expression of VSIG4 is associated with poor prognosis in hepatitis B virus-related HCC patients. Dove Medical Press 2018-09-20 /pmc/articles/PMC6159793/ /pubmed/30288101 http://dx.doi.org/10.2147/CMAR.S165822 Text en © 2018 Zhu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Zhu, Sicong Tan, Wenliang Li, Wenxin Zhou, Rui Wu, Xiaolin Chen, Xianqing Li, Wenda Shang, Changzhen Chen, Yajin Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection |
title | Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection |
title_full | Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection |
title_fullStr | Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection |
title_full_unstemmed | Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection |
title_short | Low expression of VSIG4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis B infection |
title_sort | low expression of vsig4 is associated with poor prognosis in hepatocellular carcinoma patients with hepatitis b infection |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159793/ https://www.ncbi.nlm.nih.gov/pubmed/30288101 http://dx.doi.org/10.2147/CMAR.S165822 |
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