CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line

We used the CRISPR/Cas9 system to knock-in reporter transgenes at the kidney injury molecule-1 (KIM-1) locus and isolated human proximal tubule cell (HK-2) clones. PCR verified targeted knock-in of the luciferase and eGFP reporter at the KIM-1 locus. HK-2-KIM-1 reporter cells responded to various st...

Descripción completa

Detalles Bibliográficos
Autores principales: Veach, Ruth Ann, Wilson, Matthew H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160069/
https://www.ncbi.nlm.nih.gov/pubmed/30260998
http://dx.doi.org/10.1371/journal.pone.0204487

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160069/
https://www.ncbi.nlm.nih.gov/pubmed/30260998
http://dx.doi.org/10.1371/journal.pone.0204487

Ejemplares similares