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Distribution of CD4(+) and CD8(+) exhausted tumor-infiltrating lymphocytes in molecular subtypes of Chinese breast cancer patients

PURPOSE: Breast cancer (BC) is the leading cancer affecting Chinese women; however, the immune microenvironment between molecular subtypes is less reported. This study aimed to investigate the distribution of tumor-infiltrating lymphocyte (TIL) subpopulations, especially exhausted CD4(+) and CD8(+)...

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Detalles Bibliográficos
Autores principales: Shi, Feng, Chang, Hong, Zhou, Quan, Zhao, Yan-Jie, Wu, Guang-Jiang, Song, Qing-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160273/
https://www.ncbi.nlm.nih.gov/pubmed/30288049
http://dx.doi.org/10.2147/OTT.S168057
Descripción
Sumario:PURPOSE: Breast cancer (BC) is the leading cancer affecting Chinese women; however, the immune microenvironment between molecular subtypes is less reported. This study aimed to investigate the distribution of tumor-infiltrating lymphocyte (TIL) subpopulations, especially exhausted CD4(+) and CD8(+) TILs in Chinese BC patients. PATIENTS AND METHODS: A total of 133 patients with breast invasive ductal carcinoma were recruited consecutively from January 1, 2012 to December 31, 2013, and TILs were detected in H&E-stained sections. Expression profiling of PD-1, CD4, and CD8 was determined by immunohistochemistry on 4 µm formalin-fixed paraffin-embedded tissue sections. The distribution of TILs was analyzed based on hormone receptor status and molecular subtypes. RESULTS: PD-1(+), CD4(+), and CD8(+) TILs distributed differently based on molecular subtypes. Compared to Luminal A, triple-negative breast cancer (TNBC) patients had more PD-1(+) TILs (39/high-power field [HPF] vs 11/HPF), PD-1(+) helper T (CD4(+)) cells (28/HPF vs 10/HPF), and PD-1(+) cytotoxic (CD8(+)) T-cells (3/HPF vs 2/HPF). CONCLUSION: TILs are distributed differently based on molecular subtypes. TNBC patients exhibit more PD-1(+) exhausted TILs, representing an inhibitory immune microenvironment. PD-1/PD-L1 pathway is a potential therapeutic target of TNBC.