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Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway

Little is known about the function of Keratin 80 (KRT80), an epithelial keratin, in cancer. This study investigated the role of KRT80 in the prognosis of colorectal carcinoma (CRC) and the underlying mechanisms involved in CRC migration and invasion. We analyzed the expression of KRT80 using The Can...

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Autores principales: Li, Changcan, Liu, Xisheng, Liu, Yuan, Liu, Xueni, Wang, Rangrang, Liao, Jianhua, Wu, Shaohan, Fan, Junwei, Peng, Zhihai, Li, Bin, Wang, Zhaowen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160410/
https://www.ncbi.nlm.nih.gov/pubmed/30262880
http://dx.doi.org/10.1038/s41419-018-1030-y
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author Li, Changcan
Liu, Xisheng
Liu, Yuan
Liu, Xueni
Wang, Rangrang
Liao, Jianhua
Wu, Shaohan
Fan, Junwei
Peng, Zhihai
Li, Bin
Wang, Zhaowen
author_facet Li, Changcan
Liu, Xisheng
Liu, Yuan
Liu, Xueni
Wang, Rangrang
Liao, Jianhua
Wu, Shaohan
Fan, Junwei
Peng, Zhihai
Li, Bin
Wang, Zhaowen
author_sort Li, Changcan
collection PubMed
description Little is known about the function of Keratin 80 (KRT80), an epithelial keratin, in cancer. This study investigated the role of KRT80 in the prognosis of colorectal carcinoma (CRC) and the underlying mechanisms involved in CRC migration and invasion. We analyzed the expression of KRT80 using The Cancer Genome Atlas and Oncomine databases. Higher expression of KRT80 was found to be significantly associated with multiple pathological parameters, lower disease-free survival, and overall survival in CRC patients. Also, KRT80 was an independent prognostic indicator for CRC. Furthermore, altered KRT80 expression impacted migration and invasion of CRC cells, as well as the expression of epithelial–mesenchymal transition (EMT)-related markers and cell morphology via the AKT pathway. Inhibiting the expression of AKT could reverse these phenomena. Liquid Chromatograph Mass Spectrometer/Mass Spectromete, Co-immunoprecipitation, and laser scanning confocal microscopy techniques showed that KRT80 could interact with protein kinase, DNA-activated, catalytic polypeptide (PRKDC). Suppressing PRKDC could inhibit the expression of AKT and EMT, as well as the migration and invasion of CRC cells. Taken together, these results demonstrated that KRT80 was an independent prognostic biomarker for CRC and promoted CRC migration and invasion by interacting with PRKDC via activation of the AKT pathway.
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spelling pubmed-61604102018-10-01 Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway Li, Changcan Liu, Xisheng Liu, Yuan Liu, Xueni Wang, Rangrang Liao, Jianhua Wu, Shaohan Fan, Junwei Peng, Zhihai Li, Bin Wang, Zhaowen Cell Death Dis Article Little is known about the function of Keratin 80 (KRT80), an epithelial keratin, in cancer. This study investigated the role of KRT80 in the prognosis of colorectal carcinoma (CRC) and the underlying mechanisms involved in CRC migration and invasion. We analyzed the expression of KRT80 using The Cancer Genome Atlas and Oncomine databases. Higher expression of KRT80 was found to be significantly associated with multiple pathological parameters, lower disease-free survival, and overall survival in CRC patients. Also, KRT80 was an independent prognostic indicator for CRC. Furthermore, altered KRT80 expression impacted migration and invasion of CRC cells, as well as the expression of epithelial–mesenchymal transition (EMT)-related markers and cell morphology via the AKT pathway. Inhibiting the expression of AKT could reverse these phenomena. Liquid Chromatograph Mass Spectrometer/Mass Spectromete, Co-immunoprecipitation, and laser scanning confocal microscopy techniques showed that KRT80 could interact with protein kinase, DNA-activated, catalytic polypeptide (PRKDC). Suppressing PRKDC could inhibit the expression of AKT and EMT, as well as the migration and invasion of CRC cells. Taken together, these results demonstrated that KRT80 was an independent prognostic biomarker for CRC and promoted CRC migration and invasion by interacting with PRKDC via activation of the AKT pathway. Nature Publishing Group UK 2018-09-27 /pmc/articles/PMC6160410/ /pubmed/30262880 http://dx.doi.org/10.1038/s41419-018-1030-y Text en © The Author(s) 2018 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Li, Changcan
Liu, Xisheng
Liu, Yuan
Liu, Xueni
Wang, Rangrang
Liao, Jianhua
Wu, Shaohan
Fan, Junwei
Peng, Zhihai
Li, Bin
Wang, Zhaowen
Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway
title Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway
title_full Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway
title_fullStr Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway
title_full_unstemmed Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway
title_short Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway
title_sort keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with prkdc via activating the akt pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160410/
https://www.ncbi.nlm.nih.gov/pubmed/30262880
http://dx.doi.org/10.1038/s41419-018-1030-y
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