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Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160465/ https://www.ncbi.nlm.nih.gov/pubmed/30262832 http://dx.doi.org/10.1038/s41598-018-32758-8 |
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author | Yudasaka, Masako Yomogida, Yohei Zhang, Minfang Nakahara, Masako Kobayashi, Norihiko Tanaka, Takeshi Okamatsu-Ogura, Yuko Saeki, Kumiko Kataura, Hiromichi |
author_facet | Yudasaka, Masako Yomogida, Yohei Zhang, Minfang Nakahara, Masako Kobayashi, Norihiko Tanaka, Takeshi Okamatsu-Ogura, Yuko Saeki, Kumiko Kataura, Hiromichi |
author_sort | Yudasaka, Masako |
collection | PubMed |
description | Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/CT) using (18)F-fluorodeoxy glucose ((18)F-FDG) or single-photon-emission computed tomography-computed tomography (SPECT/CT) using [(123)/(125)I]-beta-methyl-p-iodophenyl-pentadecanoic acid. Although sympathetic nerve activity and thermogenic capacity of BA is downregulated under fasting conditions in mice, fasting-dependent structural changes and fluid kinetics of BAT remain unknown. Here we show that the fasting induces fine and reversible structural changes in the non-parenchymal region in murine BAT with widened intercellular spaces and deformed collagen bands as revealed by electron microscopy. Interestingly, a newly introduced near infrared fluorescent probe of single-walled carbon nanotubes (CNTs) coated with phospholipid polyethylene glycol (PLPEG) easily demonstrated enhanced vascular permeability in BAT by the fasting. PLPEG-CNTs extravasated and remained in intercellular spaces or further redistributed in parenchymal cells in fasted mice, which is a previously unknown phenomenon. Thus, PLPEG-CNTs provide a powerful tool to trace fluid kinetics in sub-tissue levels. |
format | Online Article Text |
id | pubmed-6160465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61604652018-09-28 Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes Yudasaka, Masako Yomogida, Yohei Zhang, Minfang Nakahara, Masako Kobayashi, Norihiko Tanaka, Takeshi Okamatsu-Ogura, Yuko Saeki, Kumiko Kataura, Hiromichi Sci Rep Article Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/CT) using (18)F-fluorodeoxy glucose ((18)F-FDG) or single-photon-emission computed tomography-computed tomography (SPECT/CT) using [(123)/(125)I]-beta-methyl-p-iodophenyl-pentadecanoic acid. Although sympathetic nerve activity and thermogenic capacity of BA is downregulated under fasting conditions in mice, fasting-dependent structural changes and fluid kinetics of BAT remain unknown. Here we show that the fasting induces fine and reversible structural changes in the non-parenchymal region in murine BAT with widened intercellular spaces and deformed collagen bands as revealed by electron microscopy. Interestingly, a newly introduced near infrared fluorescent probe of single-walled carbon nanotubes (CNTs) coated with phospholipid polyethylene glycol (PLPEG) easily demonstrated enhanced vascular permeability in BAT by the fasting. PLPEG-CNTs extravasated and remained in intercellular spaces or further redistributed in parenchymal cells in fasted mice, which is a previously unknown phenomenon. Thus, PLPEG-CNTs provide a powerful tool to trace fluid kinetics in sub-tissue levels. Nature Publishing Group UK 2018-09-27 /pmc/articles/PMC6160465/ /pubmed/30262832 http://dx.doi.org/10.1038/s41598-018-32758-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yudasaka, Masako Yomogida, Yohei Zhang, Minfang Nakahara, Masako Kobayashi, Norihiko Tanaka, Takeshi Okamatsu-Ogura, Yuko Saeki, Kumiko Kataura, Hiromichi Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes |
title | Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes |
title_full | Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes |
title_fullStr | Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes |
title_full_unstemmed | Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes |
title_short | Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes |
title_sort | fasting-dependent vascular permeability enhancement in brown adipose tissues evidenced by using carbon nanotubes as fluorescent probes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160465/ https://www.ncbi.nlm.nih.gov/pubmed/30262832 http://dx.doi.org/10.1038/s41598-018-32758-8 |
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