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Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes

Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/...

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Autores principales: Yudasaka, Masako, Yomogida, Yohei, Zhang, Minfang, Nakahara, Masako, Kobayashi, Norihiko, Tanaka, Takeshi, Okamatsu-Ogura, Yuko, Saeki, Kumiko, Kataura, Hiromichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160465/
https://www.ncbi.nlm.nih.gov/pubmed/30262832
http://dx.doi.org/10.1038/s41598-018-32758-8
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author Yudasaka, Masako
Yomogida, Yohei
Zhang, Minfang
Nakahara, Masako
Kobayashi, Norihiko
Tanaka, Takeshi
Okamatsu-Ogura, Yuko
Saeki, Kumiko
Kataura, Hiromichi
author_facet Yudasaka, Masako
Yomogida, Yohei
Zhang, Minfang
Nakahara, Masako
Kobayashi, Norihiko
Tanaka, Takeshi
Okamatsu-Ogura, Yuko
Saeki, Kumiko
Kataura, Hiromichi
author_sort Yudasaka, Masako
collection PubMed
description Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/CT) using (18)F-fluorodeoxy glucose ((18)F-FDG) or single-photon-emission computed tomography-computed tomography (SPECT/CT) using [(123)/(125)I]-beta-methyl-p-iodophenyl-pentadecanoic acid. Although sympathetic nerve activity and thermogenic capacity of BA is downregulated under fasting conditions in mice, fasting-dependent structural changes and fluid kinetics of BAT remain unknown. Here we show that the fasting induces fine and reversible structural changes in the non-parenchymal region in murine BAT with widened intercellular spaces and deformed collagen bands as revealed by electron microscopy. Interestingly, a newly introduced near infrared fluorescent probe of single-walled carbon nanotubes (CNTs) coated with phospholipid polyethylene glycol (PLPEG) easily demonstrated enhanced vascular permeability in BAT by the fasting. PLPEG-CNTs extravasated and remained in intercellular spaces or further redistributed in parenchymal cells in fasted mice, which is a previously unknown phenomenon. Thus, PLPEG-CNTs provide a powerful tool to trace fluid kinetics in sub-tissue levels.
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spelling pubmed-61604652018-09-28 Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes Yudasaka, Masako Yomogida, Yohei Zhang, Minfang Nakahara, Masako Kobayashi, Norihiko Tanaka, Takeshi Okamatsu-Ogura, Yuko Saeki, Kumiko Kataura, Hiromichi Sci Rep Article Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/CT) using (18)F-fluorodeoxy glucose ((18)F-FDG) or single-photon-emission computed tomography-computed tomography (SPECT/CT) using [(123)/(125)I]-beta-methyl-p-iodophenyl-pentadecanoic acid. Although sympathetic nerve activity and thermogenic capacity of BA is downregulated under fasting conditions in mice, fasting-dependent structural changes and fluid kinetics of BAT remain unknown. Here we show that the fasting induces fine and reversible structural changes in the non-parenchymal region in murine BAT with widened intercellular spaces and deformed collagen bands as revealed by electron microscopy. Interestingly, a newly introduced near infrared fluorescent probe of single-walled carbon nanotubes (CNTs) coated with phospholipid polyethylene glycol (PLPEG) easily demonstrated enhanced vascular permeability in BAT by the fasting. PLPEG-CNTs extravasated and remained in intercellular spaces or further redistributed in parenchymal cells in fasted mice, which is a previously unknown phenomenon. Thus, PLPEG-CNTs provide a powerful tool to trace fluid kinetics in sub-tissue levels. Nature Publishing Group UK 2018-09-27 /pmc/articles/PMC6160465/ /pubmed/30262832 http://dx.doi.org/10.1038/s41598-018-32758-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yudasaka, Masako
Yomogida, Yohei
Zhang, Minfang
Nakahara, Masako
Kobayashi, Norihiko
Tanaka, Takeshi
Okamatsu-Ogura, Yuko
Saeki, Kumiko
Kataura, Hiromichi
Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
title Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
title_full Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
title_fullStr Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
title_full_unstemmed Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
title_short Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes
title_sort fasting-dependent vascular permeability enhancement in brown adipose tissues evidenced by using carbon nanotubes as fluorescent probes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160465/
https://www.ncbi.nlm.nih.gov/pubmed/30262832
http://dx.doi.org/10.1038/s41598-018-32758-8
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