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In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus

The thermophilic, endospore-forming genus of Geobacillus has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus t...

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Detalles Bibliográficos
Autores principales: Egan, Kevin, Field, Des, Ross, R. Paul, Cotter, Paul D., Hill, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160750/
https://www.ncbi.nlm.nih.gov/pubmed/30298056
http://dx.doi.org/10.3389/fmicb.2018.02116
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author Egan, Kevin
Field, Des
Ross, R. Paul
Cotter, Paul D.
Hill, Colin
author_facet Egan, Kevin
Field, Des
Ross, R. Paul
Cotter, Paul D.
Hill, Colin
author_sort Egan, Kevin
collection PubMed
description The thermophilic, endospore-forming genus of Geobacillus has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus that has not been fully explored or realized is its use as a source of novel forms of the ribosomally synthesized antimicrobial peptides known as bacteriocins. To date only two bacteriocins have been fully characterized within this genus, i.e., Geobacillin I and II, with only a small number of others partially characterized. Here we bioinformatically investigate the potential of this genus as a source of novel bacteriocins through the use of the in silico screening software BAGEL3, which scans publically available genomes for potential bacteriocin gene clusters. In this study we examined the association of bacteriocin gene presence with niche and phylogenetic position within the genus. We also identified a number of candidates from multiple bacteriocin classes which may be promising antimicrobial candidates when investigated in vitro in future studies.
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spelling pubmed-61607502018-10-08 In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus Egan, Kevin Field, Des Ross, R. Paul Cotter, Paul D. Hill, Colin Front Microbiol Microbiology The thermophilic, endospore-forming genus of Geobacillus has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus that has not been fully explored or realized is its use as a source of novel forms of the ribosomally synthesized antimicrobial peptides known as bacteriocins. To date only two bacteriocins have been fully characterized within this genus, i.e., Geobacillin I and II, with only a small number of others partially characterized. Here we bioinformatically investigate the potential of this genus as a source of novel bacteriocins through the use of the in silico screening software BAGEL3, which scans publically available genomes for potential bacteriocin gene clusters. In this study we examined the association of bacteriocin gene presence with niche and phylogenetic position within the genus. We also identified a number of candidates from multiple bacteriocin classes which may be promising antimicrobial candidates when investigated in vitro in future studies. Frontiers Media S.A. 2018-09-20 /pmc/articles/PMC6160750/ /pubmed/30298056 http://dx.doi.org/10.3389/fmicb.2018.02116 Text en Copyright © 2018 Egan, Field, Ross, Cotter and Hill. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Egan, Kevin
Field, Des
Ross, R. Paul
Cotter, Paul D.
Hill, Colin
In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
title In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
title_full In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
title_fullStr In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
title_full_unstemmed In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
title_short In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus
title_sort in silico prediction and exploration of potential bacteriocin gene clusters within the bacterial genus geobacillus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160750/
https://www.ncbi.nlm.nih.gov/pubmed/30298056
http://dx.doi.org/10.3389/fmicb.2018.02116
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