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Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China

Abstract. To provide markers to identify chromosomes in the genome of octopods, chromosomes of three octopus species were subjected to NOR/C-banding. In addition, we examined their genome size (C value) to submit it to the Animal Genome Size Database. Silver staining revealed that the number of Ag-n...

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Autores principales: Wang, Jin-hai, Zheng, Xiao-dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160780/
https://www.ncbi.nlm.nih.gov/pubmed/30275929
http://dx.doi.org/10.3897/CompCytogen.v12i3.25462
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author Wang, Jin-hai
Zheng, Xiao-dong
author_facet Wang, Jin-hai
Zheng, Xiao-dong
author_sort Wang, Jin-hai
collection PubMed
description Abstract. To provide markers to identify chromosomes in the genome of octopods, chromosomes of three octopus species were subjected to NOR/C-banding. In addition, we examined their genome size (C value) to submit it to the Animal Genome Size Database. Silver staining revealed that the number of Ag-nucleoli was 2 (Octopusminor (Sasaki, 1920)), 2 (Amphioctopusfangsiao (d’Orbigny, 1839)) and 1 (Cistopuschinensis Zheng et al., 2012), respectively, and the number of Ag-nucleoli visible was the same as that of Ag-NORs on metaphase plates in the same species. In all analyzed metaphases, Ag-NORs were mainly located terminally on the long arms of chromosomes 3 (3(rd)) of O.minor and on the short arms of chromosomes 4 (4(th)) of A.fangsiao, whereas only one of the chromosomes 23 (23(rd)) was found Ag-NORs of C.chinensis. C-bands were localized predominantly in the centromeric regions of chromosomes in the three species, while other conspicuous stable C-bands were observed in terminal regions, including the Ag-NORs. That means these three chromosome pairs (3(rd), 4(th) and 23(rd)) could be considered species-specific cytogenetic markers. The mean C values of O.minor, A.fangsiao and C.chinensis were 7.81±0.39 pg (0.070 pg per unit length), 8.31±0.18 pg (0.068 pg per unit length) and 5.29±0.10 pg (0.038 pg per unit length), respectively, and results showed that C values of the three species were not proportional to the relative length of the chromosomes. These cytogenetic characteristics will provide more theoretical foundation for further researches on chromosome evolution in octopods.
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spelling pubmed-61607802018-10-01 Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China Wang, Jin-hai Zheng, Xiao-dong Comp Cytogenet Research Article Abstract. To provide markers to identify chromosomes in the genome of octopods, chromosomes of three octopus species were subjected to NOR/C-banding. In addition, we examined their genome size (C value) to submit it to the Animal Genome Size Database. Silver staining revealed that the number of Ag-nucleoli was 2 (Octopusminor (Sasaki, 1920)), 2 (Amphioctopusfangsiao (d’Orbigny, 1839)) and 1 (Cistopuschinensis Zheng et al., 2012), respectively, and the number of Ag-nucleoli visible was the same as that of Ag-NORs on metaphase plates in the same species. In all analyzed metaphases, Ag-NORs were mainly located terminally on the long arms of chromosomes 3 (3(rd)) of O.minor and on the short arms of chromosomes 4 (4(th)) of A.fangsiao, whereas only one of the chromosomes 23 (23(rd)) was found Ag-NORs of C.chinensis. C-bands were localized predominantly in the centromeric regions of chromosomes in the three species, while other conspicuous stable C-bands were observed in terminal regions, including the Ag-NORs. That means these three chromosome pairs (3(rd), 4(th) and 23(rd)) could be considered species-specific cytogenetic markers. The mean C values of O.minor, A.fangsiao and C.chinensis were 7.81±0.39 pg (0.070 pg per unit length), 8.31±0.18 pg (0.068 pg per unit length) and 5.29±0.10 pg (0.038 pg per unit length), respectively, and results showed that C values of the three species were not proportional to the relative length of the chromosomes. These cytogenetic characteristics will provide more theoretical foundation for further researches on chromosome evolution in octopods. Pensoft Publishers 2018-09-04 /pmc/articles/PMC6160780/ /pubmed/30275929 http://dx.doi.org/10.3897/CompCytogen.v12i3.25462 Text en Jin-hai Wang, Xiao-dong Zheng http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Jin-hai
Zheng, Xiao-dong
Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China
title Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China
title_full Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China
title_fullStr Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China
title_full_unstemmed Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China
title_short Cytogenetic studies in three octopods, Octopusminor, Amphioctopusfangsiao, and Cistopuschinensis from the coast of China
title_sort cytogenetic studies in three octopods, octopusminor, amphioctopusfangsiao, and cistopuschinensis from the coast of china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160780/
https://www.ncbi.nlm.nih.gov/pubmed/30275929
http://dx.doi.org/10.3897/CompCytogen.v12i3.25462
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